Effects of three fluid resuscitation methods on apoptosis of visceral organs in rats with hemorrhagic shock |
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| Authors: | Lu Yuan-qiang Cai Xiu-jun Gu Lin-hui Fan Yu-jing Wang Qi and Bao De-guo |
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| Institution: | (1) Department of Emergency, Sir Run Run Shaw Hospital, School of Medicine, Zhejiang University, 310016 Hangzhou, China;(2) Cancer Institute, Zhejiang Tumor Hospital, 310022 Hangzhou, China |
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| Abstract: | Objective: To observe the effects of three fluid resuscitation methods on apoptosis of visceral organs in rats with hemorrhagic
shock. Methods: A model of rat with severe hemorrhagic shock and active bleeding was established in 32 SD (Sprague-Dawley)
rats. The rats were randomly divided into control group, no fluid resuscitation group (NF group), controlled fluid resuscitation
group (NS40 group) and rapid large scale fluid resuscitation group (NS80 group). Each group contained 8 rats. The curative
effects were compared. At the same time, the apoptosis in liver, kidney, lung and small intestinal mucosa of survivors after
hemorrhage and resuscitation was detected by light microscopy in HE (hematoxylin and eosin) stained tissue sections, flow
cytometry and terminal deoxynucleotidyl transferase dUTP nick end labelling (TUNEL). Results: The survival rate of early fluid
resuscitation (14/16) was markedly higher than that of NF group (3/8). There was some apoptosis in liver, kidney, lung and
small intestinal mucosa of all survivors. Compared with NF and NS40 groups, the apoptosis of liver, kidney and small intestinal
mucosa of NS80 group was obviously increased. Conclusions: Among three fluid resuscitation methods, controlled fluid resuscitation
can obviously improve the early survival rate and the apoptosis of liver, kidney and small intestinal mucosa in rats with
severe and uncontrolled hemorrhagic shock, and may benefit improvement of prognosis. |
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| Keywords: | Shock Hemorrhagic Resuscitation Apoptosis |
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