Albumin resuscitation protects against traumatic/hemorrhagic shock-induced lung apoptosis in rats |
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| Authors: | Yun Zhang Zhong-yan Liang Shao-yang Zhang Fang-fang Huang Wei Wu Yuan Gao Zuo-bing Chen |
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| Institution: | [1]Department of Emergency Surgery, the First Affiliated Hospital, School of Medicine, Zhejiang University, Hangzhou 310003, China [2]Department of Intensive Care Unit, the First Affiliated Hospital, School of Medicine, Zhejiang University, Hangzhou 310003, China [3]Institute of lnfectious Diseases, the First Affiliated Hospital School of Medicine, Zhejiang University, Hangzhou 310003, China |
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| Abstract: | Objective: To determine the effects of albumin administration on lung injury and apoptosis in traumatic/hemorrhagic shock (T/HS) rats. Methods: Studies were performed on an in vivo model of spontaneously breathing rats with induced T/HS; the rats were subjected to femur fracture, ischemia for 30 min, and reperfusion for 20 rain with Ringer's lactate solution (RS) or 5% (w/v) albumin (ALB), and the left lower lobes of the lungs were resected. Results: Albumin administered during reperfusion markedly attenuated injury of the lung and decreased the concentration of lactic acid and the number of in situ TdT-mediated dUTP nick-end labelling (TUNEL)-positive cells. Moreover, immunohistochemistry performed 24 h after reperfusion revealed increases in the level of nuclear factor κB (NF-κB), and phosphorylated p38 mitogen-activated protein kinase (MAPK) in the albumin-untreated group was down-regulated by albumin treatment when compared with the sham rats. Conclusion: Resuscitation with albumin attenuates tissue injury and inhibits T/HS-induced apoptosis in the lung via the p38 MAPK signal transduction pathway that functions to stimulate the activation of NF-κB. |
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| Keywords: | Traumatic/hemorrhagic shock (T/HS) Mitogen-activated protein kinase (MAPK) Albumin Apoptosis |
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