Monoclonal antibody-based ELISA and colloidal gold-based immunochromatographic assay for streptomycin residue detection in milk and swine urine |
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| Authors: | Jian-Xiang Wu Shao-en Zhang Xue-ping Zhou |
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| Institution: | 1. Institute of Biotechnology, Zhejiang University, Hangzhou, 310029, China
2. Nankai Biotechnology Limited Company, Hangzhou, 310051, China
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| Abstract: | A protein conjugate of streptomycin (streptomycin-bovine serum albumin (BSA) conjugate) was prepared and used as immunogen to produce monoclonal antibodies (MAb). One hybridoma secreting anti-streptomycin MAb was obtained and then used to produce MAb. The MAb named 13H5 showed the 50% maximal inhibitory concentration (IC50) value of 4.65 ng/ml and the IC20 value of 0.21 ng/ml in phosphate buffered saline (PBS). At optimum conditions, an indirect competitive enzyme-linked immunosorbent assay (ELISA) and a co... |
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| Keywords: | Streptomycin Monoclonal antibody Antibiotic residue Enzyme-linked immunosorbent assay (ELISA) Colloidal gold-based immunochromatographic assay (CGIA) |
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