| 凝胶电泳法对杂交稻种子真实性及品种纯度的鉴定 |
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| 引用本文： | 徐志敏,张连科.凝胶电泳法对杂交稻种子真实性及品种纯度的鉴定[J].陕西理工学院学报(社会科学版),2002(3):92-95. |
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| 作者姓名： | 徐志敏 张连科 |
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| 作者单位： | 陕西省汉中农业学校 陕西汉中723001
(徐志敏)，陕西省汉中农业学校 陕西汉中723001(张连科) |
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| 摘 要： | 利用聚丙酰胺凝胶电泳法鉴定不同杂交稻种子脂酶同功酶差别 ,以达到鉴定杂交稻种子真实性及品种纯度的目的 ,并以田间种植鉴定结果验证 ,诣在探索该方法的可靠性、准确性及重演性 ,为实践应用提供依据 ,取得了较好效果 .另外 ,通过不同材料脂酶同功酶酶谱比较 ,可以发现其亲缘关系 ;可以作为植物分类研究及杂种优势预测的重要依据
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| 关 键 词： | 脂酶同功梅 杂交稻 种子真实性 品种纯度 |
A Determination Report of the Authenticity of the Hybrid-rice Seeds and the Variety Purity by Means of PAM Gel Electrophoresis |
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| XU Zhi_min,ZHANG Lian_ke Hanzhong Agricultural School,Hanzhong ,Shaanxi,China.A Determination Report of the Authenticity of the Hybrid-rice Seeds and the Variety Purity by Means of PAM Gel Electrophoresis[J].Journal of Shaanxi University of Technology:Social Sciences,2002(3):92-95. |
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| Authors: | XU Zhi_min ZHANG Lian_ke Hanzhong Agricultural School Hanzhong Shaanxi China |
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| Institution: | XU Zhi_min,ZHANG Lian_ke Hanzhong Agricultural School,Hanzhong 723001,Shaanxi,China) |
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| Abstract: | Through the methods of plyacryloamide (PAM) gel electro phoresis, this article has determined the difference of lipase_isoenzyme in diff erent hybrid_rice seeds and achieved the final determination of its authenticity and the variety purity. And furtherly it was tests and verified by results of f ield planting so as to prove the methods reliability,accuracy and recurrence, wh ich provides a scientific basis for practice and application and reached satisfa ctory effects. Besides, by comparing the pedigrees of different lipase _isoenzym e, the close relationships between them can be found out so that it can be used as an important foundation for plant taxonomy research and heterosis forecast. |
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| Keywords: | lipase_isoenzyme hybrid_rice seeds autenticity of s eeds variety purity |
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