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Diagnostic Value of PCR in Genitourinary Tuberculosis |
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| Authors: | Narotam Sharma Veena Sharma Prem Raj Singh Shivani Sailwal Rajeev S Kushwaha Rajesh K Singh Satish C Nautiyal Pankaj Mishra Tariq Masood R K Singh |
| Institution: | 1. Molecular Research Laboratory, Department of Biochemistry, Shri Guru Ram Rai Institute of Medical & Health Sciences (SGRRIM&HS), Patel Nagar, Dehradun, 248001, Uttarakhand, India 3. Department of Bioscience and Biotechnology, Banasthali University, Banasthali, 304022, Rajasthan, India 2. Department of Community Medicine, Shri Guru Ram Rai Institute of Medical & Health Sciences (SGRRIM&HS), Patel Nagar, Dehradun, 248001, Uttarakhand, India |
| Abstract: | Genitourinary tuberculosis is a disease of the genitourinary system which includes the entire urinary tract and reproductive system. Genital tuberculosis is an important cause of female infertility, especially in developing nations like India. In the present study, a total of 257 clinical specimens comprising of endometrial biopsy (109), endometrial curetting (42), menstrual blood (8), semen (17), placenta (11) and urine (70) were collected from patients and subjected for PCR, Culture and AFB detection. The endometrial biopsy, endometrial curetting, menstrual blood, semen, placenta, urine showed 30.2, 45.2,12.5, 5.8, 27.2, 31.4 %, positivity rate for tuberculosis by PCR, 7.3, 9.5, 25.0, 0, 9, 8.5 % by culture and 1.8, 2.3, 0, 0, 0, 2.8 % respectively by AFB smear. Being a novel, rapid technique, PCR is the method of choice for rapid diagnosis and management of genitourinary tuberculosis shared with the other concerned tests. This study reveals that genital tuberculosis can occur in any age group, however, the majority of patients were from reproductive age (nearly 75 % of them were from 20–45 years of age) group. |
| Keywords: | Polymerase chain reaction MPB64 Bactec 460 Genitourinary tuberculosis |
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