| Immobilization of Aspergillus Oryzae Mycelium Pellets and Its Application in the Resolution of D, L-Alanine |
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| 作者姓名： | 王燕 朱怀工 张凤宝 王淑兰 蔡旺锋 张旭斌 |
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| 作者单位： | School of Chemical Engineering and Technology Tianjin University Tianjin 300072 China，School of Chemical Engineering and Technology Tianjin University Tianjin 300072 China Tianjin Univtech Co. Ltd Tianjin 300072 China，School of Chemical Engineering and Technology Tianjin University Tianjin 300072 China，School of Chemical Engineering and Technology Tianjin University Tianjin 300072 China，School of Chemical Engineering and Technology Tianjin University Tianjin 300072 China，School of Chemical Engineering and Technology Tianjin University Tianjin 300072 China |
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| 摘 要： | Aspergillus oryzae 3042 mycelium pellets were immobilized by crosslinking method with reagents of gelatin and formaldehyde. An orthogonal design table was used to determine the optimal immobilization conditions. The L-aminoacylase activity of immobilized mycelium pellets under optimal conditions was assayed. The results show that the L-aminoacylase activity was 1 350 U·g-1. and the activity retention rate was 83%. The properties of the immobilized preparation were studied. Compared with free pellets, the appropriate pH of reaction system changed from 7.0 to 8.0, temperature changed from 52℃to 63℃, and the ranges of the optimum reaction conditions were all improved. The effect of Co2 on immobilized mycelium pellets was investigated and the favorable concentration was determined. When the immobilized preparation was used for the resolution of D, L-alanine in a packed bed reactor with 10 mm in diameter, 200 mm in height, the operational stability was increased, and the half-life period was 53 d. Thermal stability analysis shows that the immobilized pellets were more stable than the free pellets.
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| 关 键 词： | 酰化氨基酸水解酶 米曲霉 墨水 菌丝球 固定 |
| 收稿时间： | 2006-03-20 |
Immobilization of Aspergillus Oryzae Mycelium Pellets and Its Application in the Resolution of D, L-Alanine |
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| WANG Yan,ZHU Nuaigong,ZHANG Fengbao,WANG Shulan,CAI Wangfeng,ZHANG Xubin.Immobilization of Aspergillus Oryzae Mycelium Pellets and Its Application in the Resolution of D, L-Alanine[J].Transactions of Tianjin University,2006,12(4):242-247. |
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| Authors: | WANG Yan ZHU Nuaigong ZHANG Fengbao WANG Shulan CAI Wangfeng ZHANG Xubin |
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| Abstract: | Aspergillus oryzae 3042 mycelium pellets were immobilized by crosslinking method with reagents of gelatin and formaldehyde. An orthogonal design table was used to determine the optimal immobilization conditions. The L-aminoacylase activity of immobilized mycelium pellets under optiand the activity retention rate was 83%. The properties of the immobilized preparation were studied. Compared with free pellets, the appropriate pH of reaction system changed from 7.0 to 8.0, temperature changed from 52 ℃ to 63 ℃, and the ranges of the optimum reaction conditions were all improved. The effect of Co2+ on immobilized mycelium pellets was investigated and the favorable concentration was determined. When the immobilized preparation was used for the resolution of D, L-alanine in a packed bed reactor with 10 mm in diameter, 200 mm in height, the operational stabili ty was increased, and the half-life period was 53 d. Thermal stability analysis shows that the immobilized pellets were more stable than the free pellets. |
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| Keywords: | L-aminoacylase Aspergillus oryzae immobilization crosslinking alanine pH temperature |
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