Effect of microcystin-LR on protein phosphatase 2A and its function in human amniotic epithelial cells |
| |
| Authors: | Liang Jing Li Tan Zhang Ya-Li Guo Zong-Lou Xu Li-Hong |
| |
| Institution: | (1) Xiamen Marine Environmental Monitoring Center, Xiamen, 361008, China |
| |
| Abstract: | Due to their toxicity, the increased distribution of microcystins (MCs) has become an important worldwide problem. MCs have
been recognized as inhibitors of protein phosphatase 2A (PP2A) through their binding to the PP2A catalytic subunit. However,
the exact mechanism of MC toxicity has not been elucidated, especially concerning the cellular response and its autoregulation.
To further dissect the role of PP2A in MC-induced toxicity, the present study was undertaken to determine the response of
PP2A in human amniotic epithelial (FL) cells treated with microcystin-LR (MCLR), one of the MC congeners. The results show
that a low-dose treatment of MCLR in FL cells for 6 h induced an increase in PP2A activity, and a high-dose treatment of MCLR
for 24 h decreased the activity of PP2A, as expected. The increased mRNA and protein levels of the PP2A C subunit may explain
the increased activity of PP2A. Furthermore, MCLR altered microtubule post-translational modifications through PP2A. These
results further clarify the underlying mechanism how MCLR affects PP2A and may be helpful for elucidating the complex toxicity
of MCLR. |
| |
| Keywords: | Microcystin-LR Protein phosphatase 2A Phosphatase activity Hormesis Tubulin B55α |
| 本文献已被 PubMed SpringerLink 等数据库收录！ |
|
