Monitoring the dielectric response of single cells following mitochondrial adenosine triphosphate synthase inhibition by oligomycin using a dielectrophoretic cytometer |
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| Authors: | B Saboktakin Rizi K Braasch E Salimi M Butler G E Bridges D J Thomson |
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| Institution: | 1.Department of Electrical and Computer Engineering, University of Manitoba, Winnipeg, Manitoba R3T5V6, Canada;2.Department of Microbiology, University of Manitoba, Winnipeg, Manitoba R3T5V6, Canada |
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| Abstract: | One of the main uses of adenosine triphosphate (ATP) within mammalian cells is powering the Na+/K+ ATPase pumps used to maintain ion concentrations within the cell. Since ion concentrations determine the cytoplasm conductivity, ATP concentration is expected to play a key role in controlling the cytoplasm conductivity. The two major ATP production pathways within cells are via glycolysis within the cytoplasm and via the electron transport chain within the mitochondria. In this work, a differential detector combined with dielectrophoretic (DEP) translation in a microfluidic channel was employed to observe single cell changes in the cytoplasm conductivity. The DEP response was made sensitive to changes in cytoplasm conductivity by measuring DEP response versus media conductivity and using double shell models to choose appropriate frequencies and media conductivity. Dielectric response of Chinese hamster ovary (CHO) cells was monitored following inhibition of the mitochondria ATP production by treatment with oligomycin. We show that in CHO cells following exposure to oligomycin (8 μg/ml) the cytoplasm conductivity drops, with the majority of the change occurring within 50 min. This work demonstrates that dielectric effects due to changes in ATP production can be observed at the single cell level. |
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