| 力竭运动后不同时相大鼠心肌Egr-1蛋白的变化及其在运动性心肌微损伤发生中的作用 |
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| 引用本文： | 袁旭鑫,常芸.力竭运动后不同时相大鼠心肌Egr-1蛋白的变化及其在运动性心肌微损伤发生中的作用[J].体育科研,2012,33(4):30-34. |
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| 作者姓名： | 袁旭鑫 常芸 |
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| 作者单位： | 国家体育总局体育科学研究所,北京体育馆路11号,北京100061 |
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| 基金项目： | 国家体育总局体育科学研究所基本科研业务经费 |
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| 摘 要： | 目的：探讨力竭运动后不同时相心肌早期生长应答基因Egr-1在蛋白水平的变化特点,为运动性心肌微损伤及心肌功能异常发生机制的阐释提供实验依据。方法：100只健康成年雄性SD大鼠,分为一次力竭游泳运动组、两周反复力竭游泳运动组及安静对照组,分别于力竭运动后即刻、6 h、12 h及24 h取材,应用免疫荧光组化技术和图像分析方法研究大鼠心肌Egr-1蛋白含量的变化。结果：一次力竭运动组大鼠心脏各部位Egr-1蛋白含量即刻组与对照组相比显著性升高（P〈0.05）,24 h组与对照组相比显著性降低（P〈0.05）;反复力竭运动组大鼠心脏各部位Egr-1蛋白含量即刻组与对照组相比显著性升高（P〈0.05）,6 h组、12 h组、24 h组大鼠心脏各部位Egr-1蛋白含量均显著低于对照组（P〈0.05）。结论：不同力竭运动后心脏各部位Egr-1在运动后即刻明显升高,表明心肌细胞Egr-1对力竭运动刺激产生快速应激反应,可能诱导炎性细胞的趋化、聚集以及氧自由基的产生。而心脏各部位Egr-1在力竭运动后24 h内恢复到正常水平,说明力竭运动后心脏Egr-1改变属早期应激反应,且此反应属可复性改变。
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| 关 键 词： | 力竭运动 大鼠 心肌 早期生长应答基因-1（Egr-1） |
Changes of Egr-1 Protein and It's Functions in the occurrence of Athletic Myocardial Micro-damage at the Different Phases after Exhaustive Exercise |
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| Yuan Xu-xin and Chang yun.Changes of Egr-1 Protein and It's Functions in the occurrence of Athletic Myocardial Micro-damage at the Different Phases after Exhaustive Exercise[J].Sports Science Research,2012,33(4):30-34. |
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| Authors: | Yuan Xu-xin and Chang yun |
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| Institution: | (China Institute of Sport Science,Beijing 100061,China) |
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| Abstract: | Objective To explore the variation characteristics of early growth response genes Egr-1 protein level so as to provide experimental reference for the occurrence mechanism of athletic myocardial micro-damage and myocardial dysfunction.Method 100 healthy adult male SD rats were divided into three groups of one-time exhaustive swimming group,two-week repeated exhaustive swimming group and no-exercise control group.Samples were collected immediately,6 hours,12 hours and 24 hours after exhaustive exercise.Immunofluorescence technology and image analysis were adopted to study the changes of rat myocardium Egr-1 protein level.Result The immediate Egr-1 protein levels of the different parts of rat myocardium increased significantly（P〈0.05）.Samples collected at 24 hours decreased distinctly compared to those of the control group（P〈0.05）.In repeated exhaustive group,the immediate Egr-1 protein levels of the different parts of rat myocardium increased significantly（P〈0.05）.Samples of Egr-1 protein levels of the different parts of rat myocardium at 6 hours,12 hours and 24 hours were clearly lower than the control group（P〈0.05）.Conclusion Egr-1 of the different parts of heart increases immediately after the different exhaustive exercises shows that there is a rapid stress response of myocardial cell Egr-1 toward the stimulation of exhaustive exercise.This might lead to chemotaxis and aggregation of inflammatory cells and the production of OFR.Egr-1 of the different parts of heart returns normal level 24 hours after exhaustive exercise.This shows that Egr-1 after exhaustive exercise belongs to early-stage stress response and this response is reversible. |
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| Keywords: | exhaustive exercise rat myocardium early-stage growth response gene(Egr-1) |
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