| Creation of reversed phase high-performance liquid chromatographic technique to assay platelet-activating factor |
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| 作者姓名： | 杨云梅 曹红翠 徐哲荣 陈晓明 |
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| 作者单位： | First Affiliated Hospital，College of Medical Science，Zhejiang University，First Affiliated Hospital，College of Medical Science，Zhejiang University，First Affiliated Hospital，College of Medical Science，Zhejiang University，First Affiliated Hospital，College of Medical Science，Zhejiang University Hangzhou 310003，China，Hangzhou 310003，China，Hangzhou 310003，China，Hangzhou 310003，China |
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| 基金项目： | Project (No. 20020798) supported by Department of Education ofZhejiang Province,China |
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| 摘 要： | INTRODUCTION Platelet activating factor (PAF), a kind of pho-spholipids compound, synthesized and secretedmainly by neutrophilic granulocyte, platelet, is ahighly potent chemical mediator in inflammationand allergic reactions and induces microvascularleakage in several tissues (Chung, 1997). PAFs arefew in vivo, have short half life (only about 30seconds) and rapidly convert to lyso-PAF that hasno bio-activity and is both the metabolite and pre-cursor of PAF (Rao, 1998). Lyso-P…
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Creation of reversed phase high-performance liquid chromatographic technique to assay platelet-activating factor |
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| YANG Yun-mei CAO Hong-cui,XU Zhe-rong,CHEN Xiao-ming.Creation of reversed phase high-performance liquid chromatographic technique to assay platelet-activating factor[J].Journal of Zhejiang University Science,2004(6). |
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| Authors: | YANG Yun-mei CAO Hong-cui XU Zhe-rong CHEN Xiao-ming |
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| Abstract: | Objective: To establish a new assay for platelet-activating factor (PAF), to compare it with bio-assay; and to discuss its significance in some elderly people diseases such as cerebral infarction and coronary heart disease. Methods: To measure PAF levels in 100 controls, 23 elderly patients with cerebral infarction and 65 cases with coronary heart disease by reversed phase high-performance liquid chromatographic technique (rHPLC). Results: rHPLC is more convenient, sensitive, specific, and less confusing, compared with bio-assay. The level of plasma PAF in patients with cerebral infarction was higher than that in the controls (P<0.01), and in patients with coronary heart disease. Conclusion: Detection of PAF with rHPLC is more reliable and more accurate. The new assay has important significance in PAF research. |
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| Keywords: | Platelet activating factor Reversed phase high-performance liquid chromatographic technique Coronary heart disease Cerebral infarction |
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