| 不结球白菜品种自交不亲和S基因型的鉴定 |
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| 引用本文： | 杜黎明,胡天华,包崇来,朱琴妹,胡海娇,毛伟海.不结球白菜品种自交不亲和S基因型的鉴定[J].科技通报,2009,25(4):465-468,540. |
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| 作者姓名： | 杜黎明 胡天华 包崇来 朱琴妹 胡海娇 毛伟海 |
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| 作者单位： | 浙江省农业科学院蔬菜研究所,杭州,310021 |
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| 基金项目： | 浙江省科技厅一般项目 |
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| 摘 要： | 为给不结球白菜的品种配置和遗传育种提供理论基础,利用特异性PCR扩增、DNA测序和生物信息学方法鉴定了7个自交系的S基因型,PCR扩增结果表明,51-14、375-124、711-114、884-214等4个自交系的基因型为Class-Ⅰ SLG和SRK,表现为自交强不亲和.523-112和544-312的基因型为Class-ⅡSLG类型.表现为自交若不亲和,和田间试验结果一致.测序结果blast分析表明844-214 Class-Ⅰ型和Brassica rapa SLG56的相似性为99%,51-14的SRK PCR与SRK56的同源性达到100%.
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| 关 键 词： | 不结球白菜 自交不亲和性 S基因型 |
Identification of the S-genotypes of Brassica campestris ssp. Chinensis |
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| DU Liming,HU Tianhua,BAO Chonglai,ZhU Qinmei,HU Haijiao,MAO Weihai.Identification of the S-genotypes of Brassica campestris ssp. Chinensis[J].Bulletin of Science and Technology,2009,25(4):465-468,540. |
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| Authors: | DU Liming HU Tianhua BAO Chonglai ZhU Qinmei HU Haijiao MAO Weihai |
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| Abstract: | In order to provide a theoretical basis for the genetic breeding of non-heading Chinese cabbage,their Sgenotypes were identified by PCR amplification,DNA sequencing and bio-informatic analysis. PCR amplification shows that fragment was generated in 51-14、375-124、711-114,884-214 by primer Class-Ⅰ SLG and SRK. Fragment of about 1000 bp was generated in 523-112 and 544-312.DNA sequence bio-informatic analysis and BLA ST reveal that 844-214 similarity with Brassica rapa SRK56. |
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| Keywords: | non-heading Chinese cabbage self-incompatibility S-allele |
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