| Cloning and characterization of a glucose 6-phosphate/phosphate translocator from Oryza sativa |
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| 作者姓名： | 姜华武 佃蔚敏 刘非燕 吴平 |
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| 作者单位： | The State Key Laboratory of Plant Physiology and Biochemistry，College of Life sciences，The State Key Laboratory of Plant Physiology and Biochemistry，College of Life sciences，The State Key Laboratory of Plant Physiology and Biochemistry，College of Life sciences，The State Key Laboratory of Plant Physiology and Biochemistry，College of Life sciences Zhejiang University，Hangzhou 310029，China，Zhejiang University，Hangzhou 310029，China，Zhejiang University，Hangzhou 310029，China，Zhejiang University，Hangzhou 310029，China |
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| 基金项目： | ProjectsupportedbyNationalNaturalScienceFoundationofChina(No .3 983 0 2 5 0 )andNaturalScienceFoundationofZhejiangProvince(No .2A0 10 6)，China .ThenucleotidesequencedatawillappearintheGenBankunderaccessionnumberAF3 75 0 5 3 . |
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| 摘 要： | INTRODUCTIONNongreenplastidsofheterotrophictissuesarecarbohydrate importingorganellesand ,inthecaseofamyloplastsofstoragetissues,thesiteofstarchsynthesis.Investigationofwholetissuesfromavarietyofstarchsynthesizingcropplantsindicatedthathexoseunitswereimpo…
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Cloning and characterization of a glucose 6-phosphate/phosphate translocator from Oryza sativa |
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| JIANG Hua wu,DIAN Wei min,LIU Fei yanWU PingWT.,.BZ.Cloning and characterization of a glucose 6-phosphate/phosphate translocator from Oryza sativa[J].Journal of Zhejiang University Science,2003(3). |
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| Authors: | JIANG Hua wu DIAN Wei min LIU Fei yanWU Ping[WT BZ] |
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| Institution: | JIANG Hua wu,DIAN Wei min,LIU Fei yanWU Ping[WT9.,10.BZ] |
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| Abstract: | Plastids of nongreen tissues import carbon as a source of biosynthetic pathways and energy, and glucose 6 phosphate is the preferred hexose phosphate taken up by nongreen plastids. A cDNA clone encoding glucose 6 phosphate/phosphate translocator ( GPT ) was isolated from a cDNA library of immature seeds of rice and named as OsGPT . The cDNA has one uninterrupted open reading frame encoding a 42 kDa polypeptide possessing transit peptide consisting of 70 amino acid residues. The OsGPT gene maps on chromosome 8 of rice and is linked to the quantitative trait locus for 1000 grain weight. The expression of OsGPT is mainly restricted to heterotrophic tissues. These results suggest that glucose 6 phosphate imported via GPT can be used for starch biosynthesis in rice nongreen plastids. |
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| Keywords: | Glucose 6 phosphate/phosphate translocator Starch synthesis Rice ( Oryza sativa L ) |
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