| Emergence of a new satellite RNA from cucumber mosaic virus isolate P1 |
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| 作者姓名： | Sandra Pérez Alvarez 薛朝阳 周雪平 |
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| 作者单位： | Institute of Biotechnology，Zhejiang University，Institute of Biotechnology，Zhejiang University，Institute of Biotechnology，Zhejiang University Hangzhou 310029，China，Hangzhou 310029，China，Hangzhou 310029，China |
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| 基金项目： | ProjectsupportedbyresearchgrantsfromtheTeachingandResearchAwardProgramforOutstandingTeachersinHigherEducationInstitutesoftheMin
istryofEducationofChinaandbytheNationalOutstandingYouthFoundationsofNationalNaturalScienceFoundationofChina (No .3 0 12 5 0 |
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| 摘 要： | INTRODUCTIONCucumbermosaicvirus (CMV) ,thetypespeciesofthegenusCucumovirusoffamilyBro moviridae,isanisometricviruswithatripartitesingle strandedRNAgenomeofpositivepolari ty.CMVhasaparticularlywidehostrange ,in fectingmorethan 80 0speciesinover 70familieso…
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Emergence of a new satellite RNA from cucumber mosaic virus isolate P1 |
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| Abstract: | The cucumber mosaic virus (CMV) isolate P1 caused very mild symptoms on many plant species. After serial passages by mechanical inoculation over five years, CMV P1 caused severe symptoms on several tobacco cultivars and tomato. A specific band of approximately 0.3 kb in length was amplified by RT PCR with primers synthesized based on reported CMV satellite RNA (satRNA) sequences. Sequence analysis showed there were two satRNAs (Sat P1 1 and Sat P1 2). Sat P1 1 contained 335 nucleotides, and Sat P1 2 contained 394 nucleotides. These two satRNAs shared 64% overall nucleotide sequence homology, and differences between the two satRNAs included mutations as well as deletions. Sat P1 1 was identical to a satRNA (Z96099) reported in 1995 in CMV P1. Based on differences in the sequence and secondary structure between these two satRNAs, we conclude that Sat P1 2 represents the emergence of a new satellite (necrotic satellite) from attenuated satRNA populations. The possible effect of the emergence of this new satRNA is discussed. |
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| Keywords: | Cucumber mosaic virus Satellite RNA Sequence |
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