| Influence of medium components on elastase production using crudesources by Bacillus sp. EL31410 |
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| 作者姓名： | 何国庆 陈启和 张丽 刘小杰 |
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| 作者单位： | Department of Food Science and Nutrition，Zhejiang University，Hangzho u 310029，China |
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| 基金项目： | Project (No .3 0 0 0 2 4)supportedbytheZhejiangProvincialNaturalScienceFoundationofChina |
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| 摘 要： | ＩＮＴＲＯＤＵＣＴＩＯＮＡｎｉｎｃｒｅａｓｉｎｇｄｅｍａｎｄｆｒｏｍｃｏｎｓｕｍｅｒｓｆｏｒｈｅａｌｔｈｙａｎｄｌｏｗｃｏｓｔｍｅｄｉｃａｌｓｈａｓｌｅｄｔｏｔｈｅｄｅ ｖｅｌｏｐｍｅｎｔｏｆａｌｔｅｒｎａｔｉｖｅｍｅｄｉｃａｌｔｈｅｒａｐｙａｇｅｎｔｓｕ
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Influence of medium components on elastase production using crudesources by Bacillus sp. EL31410 |
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| Abstract: | A newly isolated strain EL31410, producing elastase (E.C3.4.4.7) with h igh elastolytic activity was identified as Bacillus sp. In the medium opt imizat ion, it was found that wheat bran and soybean flour hydrosate were the best crud e carbon and nitrogen source for enzyme production, respectively. Addition of co rn steep flour can affect the bacterium growth and elastase production. A fracti onal factorial design was applied to study the main factors that affect the enzy me production, and central composite experimental design and response surface me thodology were adopted to derive a statistical model for the effect of wheat bra n and soybean flour hydrosate on elastase production. The experimental results s howed that wheat bran had positive effect but soybean flour hydrosate had neg ative effect, on enzyme production. An initial concentration of 3.4%(w/v) wh eat b ran and 9.4%(v/v) soybean flour hydrosate were found to be optimal for enzyme pr oduction in batch culture. The time course of elastase production in the optimiz ed medium composition was also described. |
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| Keywords: | Elastase Bacillus sp EL31410 Crude sources Medium o ptimization Fractional factorial design (FFD) Central composite design (CCD) Response surface methodology (RSM) Batch cultivation |
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