| HPLC法测定石杉属植物中石杉碱甲的含量 |
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| 引用本文： | 王姗,王沫.HPLC法测定石杉属植物中石杉碱甲的含量[J].商洛学院学报,2012,26(2):38-41. |
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| 作者姓名： | 王姗 王沫 |
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| 作者单位： | 1. 商洛学院生物医药工程系,陕西商洛,726000
2. 华中农业大学药用植物研究所,湖北武汉,430070 |
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| 基金项目： | 国家科技支持计划(2006BA106A10-2-3) |
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| 摘 要： | 采用YWGC18柱(4.6 mm×250 mm,10μm),以甲醇(80 mmol.L-1)-醋酸铵溶液(pH 6.0)(35:65)为流动相,流速为1.0 mL.min-1;检测波长为310 nm,建立了HPLC法测定湖北利川三种石杉属植物(蛇足石杉、皱边石杉、四川石杉)中石杉碱甲含量,以期发掘替代资源。结果显示,三种石杉属植物中均含有石杉碱甲,且四川石杉最多,蛇足石杉次之,皱边石杉最少,故四川石杉可以作为提取石杉碱甲的原料药材之一,具有较高的开发和利用价值。
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| 关 键 词： | 石杉属植物 石杉碱甲 高效液相色谱法 |
Determination of Huperzine A in Three Species of Huperzia Berm.by HPLC |
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| WANG Shan,WANG Mo.Determination of Huperzine A in Three Species of Huperzia Berm.by HPLC[J].Journal of Shangluo University,2012,26(2):38-41. |
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| Authors: | WANG Shan WANG Mo |
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| Institution: | 1.Biopharmaceutical Engineering Department of Shangluo University,Shangluo,Shaanxi 726000; 2.Medicinal Institute of Huazhong Agricultural University,Wuhan,Hubei 430070) |
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| Abstract: | In order to discover the substitute resource,HPLC method was established for the determination of Huperzine A(Hup A)in 3 species from Lichuan,A YWGC18column was used with the mobile phase consisted of a mixture of methanol-ammonium acetate(35:65)at a flow rate of 1.0ml/min detected at the wavelength of 310 nm.The rusult shows that the three species are all contains Hup A,and highest content of Hup A in Huperzia sutchueniana(Herter) Ching.The lowest content in Huperzia crispate(Ching ex H.S. Kung) Ching.So Huperzia sutchueniana(Herter) Ching.can be used in production as material. |
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| Keywords: | plants of Huperzia Hupeizine A HPLC |
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