| 肉鸡IGF-I基因mRNA的时空表达模式 |
| |
| 引用本文： | 顾海娟,王星果,王慧娟,邵芳,顾志良.肉鸡IGF-I基因mRNA的时空表达模式[J].常熟理工学院学报,2014(2):13-16,22. |
| |
| 作者姓名： | 顾海娟 王星果 王慧娟 邵芳 顾志良 |
| |
| 作者单位： | 常熟理工学院生物与食品工程学院,江苏常熟215500 |
| |
| 基金项目： | 国家自然科学基金项目“生长激素调控鸡IGF-I基因表达的分子机理研究”（31072025） |
| |
| 摘 要： | 采用实时荧光定量PCR技术，以β-actin为内参，检测了白羽AA肉鸡在4周龄时其胸肌、腿肌、脂肪、心、肝、脾、肺、肾、腺胃、肌胃、肠、脑共12个组织和在1日龄及1、2、3、4、5、6、7周龄时腿肌、肝脏、脂肪中类胰岛素生长因子-I（IGF-I）的表达量变化。结果显示，除肾外其余组织均表达一定量的IGF-I mRNA表达，且在肝脏中表达量显著高于其他组织；腿肌6周时IGF-I mRNA表达量达到最大值，其余各时期也都高于1日龄时的表达；肝脏中表达量最高时期是3周，然后又呈下降趋势；脂肪中在3周时表达量最高，然后其表达量随年龄增长而逐渐下降。本实验结果揭示了生长早期肉鸡IGF-I基因mRNA的时空表达特征，为进一步研究生长轴相关基因对肉鸡早期生长发育的调控作用奠定了基础。
|
| 关 键 词： | 肉鸡 IGF-I基因 时空表达 |
The Temporal and Spatial Characteristics of IGF-I Gene Expression in Broilers |
| |
| GU Hai-Juan,WANG Xing-Guo,WANG Hui-Juan,Shao Fang,GU Zhi-Liang.The Temporal and Spatial Characteristics of IGF-I Gene Expression in Broilers[J].Journal of Changshu Institute of Technology,2014(2):13-16,22. |
| |
| Authors: | GU Hai-Juan WANG Xing-Guo WANG Hui-Juan Shao Fang GU Zhi-Liang |
| |
| Institution: | (School of Biology and Food Engineering, Changshu Institute of Technology, Changshu 215500, China) |
| |
| Abstract: | In this experiment, the real-time fluorescence quantitative PCR was applied to investigate the tissue expressional patterns of IGF-I mRNA in breast muscle, thigh muscle, adipose, heart, liver, spleen, lung, kidney, glandular stomach, muscular stomach, intestine, brain tissues and in various developmental stages （d1, w1, w2, w3, w4, w5, w6 and w7） of thigh muscle, liver, adipose tissue in three different broilers, and β-actin gene stan-dards was used as an inner control. The results showed that the expression of IGF-I gene in liver was significant-ly higher than that of the other tissues, and expressed at a certain amount of IGF-I gene in all tissues except for kidney, that the highest expression of IGF-I mRNA in thigh muscle was at age of six-week old, and the rest of the stage was also higher than one day, that the highest expression of IGF-I mRNA in liver was at three-week, and then declined, that the highest expression in adipose was at three-week, and then its expression gradually decreased with increasing age. These research results revealed the temporal and spatial expression characteris-tics of IGF-I mRNA gene in early growth broilers, and would contribute to the further study on genes related to growth axis regulation of growth and development in broilers. |
| |
| Keywords: | broilers IGF-I gene temporal and spatial characteristics of gene expression |
| 本文献已被 CNKI 维普 等数据库收录！ |
|
