Association between infection of different strains of <Emphasis Type="Italic">Porphyromonas gingivalis</Emphasis> and <Emphasis Type="Italic">Actinobacillus actinomycetemcomitans</Emphasis> in subgingival plaque and clinical parameters in chronic periodontitis |
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| Authors: | Wu Yan-min Yan Jie Chen Li-li Gu Zhi-yuan |
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| Institution: | (1) Department of Stomatology, the Second Affiliated Hospital, School of Medicine, Zhejiang University, Hangzhou, 310009, China;(2) Department of Medical Microbiology and Parasitology, School of Medicine, Zhejiang University, Hangzhou, 310009, China;(3) Department of Oral and Maxillofacial Surgery, the Affiliated Hospital of Stomatology, School of Medicine, Zhejiang University, Hangzhou, 310003, China |
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| Abstract: | Objective The aim of this study was to investigate subgingival infection frequencies of Porphyromonas gingivalis and Actinobacillus actinomycetemcomitans strains with genetic variation in Chinese chronic periodontitis (CP) patients and to evaluate its correlation with clinical
parameters.
Methods Two multiplex polymerase chain reaction (PCR) assays were developed to detect the 16Sr DNA, collagenase (prtC) and fimbria (fimA) genes of P. gingivalis and the 16Sr DNA, leukotoxin (lktA) and fimbria-associated protein (fap) genes of A. actinomycetemcomitans in 60 sulcus samples from 30 periodontal healthy subjects and in 122 subgingival plaque samples from 61 patients with CP.
The PCR products were further T-A cloned and sent for nucleotide sequence analysis.
Results The 16Sr DNA, prtC and fimA genes of P. gingivalis were detected in 92.6%, 85.2% and 80.3% of the subgingival plaque samples respectively, while the 16Sr DNA, lktA and fap genes of A. actinomycetemcomitans were in 84.4%, 75.4% and 50.0% respectively. Nucleotide sequence analysis showed 98.62%∼100% homology of the PCR products
in these genes with the reported sequences. P. gingivalis strains with prtC+/fimA+ and A. actinomycetemcomitans with lktA+ were predominant in deep pockets (>6 mm) or in sites with attachment loss ≥5 mm than in shallow pockets (3∼4 mm) or in sites
with attachment loss ≤2 mm (P<0.05). P. gingivalis strains with prtC+/fimA+ also showed higher frequency in gingival index (GI)=3 than in GI=1 group (P<0.05).
Conclusion Infection of P. gingivalis with prtC+/fimA+ and A. actinomycetemcomitans with lktA+ correlates with periodontal destruction of CP in Chinese. Nonetheless P. gingivalis fimA, prtC genes and A. actinomycetemcomitans lktA gene are closely associated with periodontal destruction, while A. actinomycetemcomitans fap gene is not.
Project (No 30471888) supported by the National Natural Science Foundation of China |
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| Keywords: | Porphyromonas gingivalis Actinobacillus actinomycetemcomitans Strain Periodontitis PCR |
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