| 红掌新品种组培快繁技术研究 |
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| 引用本文： | 廖俊杰,李进进,黄锡琼,王育林,夏时云.红掌新品种组培快繁技术研究[J].广东轻工职业技术学院学报,2003,2(1):4-6. |
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| 作者姓名： | 廖俊杰 李进进 黄锡琼 王育林 夏时云 |
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| 作者单位： | [1]广东轻工职业技术学院食品生物工程系,广东广州510300 [2]广东轻工职业技术学院教务处,广东广州510300 [3]深圳市金百合生物工程技术有限公司,深圳龙岗518122 |
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| 基金项目： | 该项目于2000年通过深圳市科技成果学鉴定（成果登记号：2000058） |
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| 摘 要： | 本试验完成了红掌新品种A1、A2、A3的组培扩繁技术路线的研究。实验发现，不同品种（基因型），叶片状态，培养基中NH4NO3浓度与叶外植体愈伤组织的诱导和芽的分化有关。在改良MS培养基中，NH4NO，400mg／L结合BA1mg／L，2，4-D0．1mg／L有利于愈伤组织形成和芽分化；以BA0．5mg／L有利于芽的增殖和继代培养；在无激素1／2MS培养基上诱导生根，生根率达100％。本技术路线已进入种苗商品化生产阶段，可以解决红掌新品种推广的种苗来源问题。
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| 关 键 词： | 红掌 快速繁殖 叶外植体 |
| 文章编号： | 1672-1950（2003）01-0004-03 |
| 收稿时间： | 2002-10-08 |
Studies on the Rapid- micropropagation in New Varieties of Anthurium Scherzerianum |
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| Liao Junjie, Li Jinjin, Huang Xiqiong, Wang Yulin, Xia Shiyun.Studies on the Rapid- micropropagation in New Varieties of Anthurium Scherzerianum[J].Journal of Guangdong Industry Technical College,2003,2(1):4-6. |
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| Authors: | Liao Junjie Li Jinjin Huang Xiqiong Wang Yulin Xia Shiyun |
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| Institution: | Department of Food and Biology Engineering, Guangdong Industry Technical College, Guangzhou 510300 ,China; 2. Teaching Affairs Division, Guangdong Industry Technical College, Guangzhou 510300, China; 3. Shenzhen Golden Lily Bio - tech. Co, Ltd, Shenzhen 518122, China |
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| Abstract: | The rapid-micropropagation technology in new varieties of anthurium scherzerianum(namely A1, A2, A3) was investigated in the experiment.It is found that the ratios of callus inducing and bud initation are related to Anthurium variety, condition of the leaf , and the NH 4NO 3 concentration in the medium. NH 4NO 3 400 mg/L , BA1 mg/L and 2,4-D0.1mg/L in the improved MS medium all contribute to callus formation and bud initation, BA0.5 mg/L stimulates to shootlets proliferation. And 100% of rooting is achieved in 1/2MS without any plant horomons. The seedlings of these new varieties were produced with the micropropagation technology and offered to the cultivative practise. |
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| Keywords: | anthurium scherzerianum rapid- micropropagation leaf explant |
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