| 一次性运动对肥胖大鼠脂肪水解调节因素的影响 |
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| 引用本文： | 田吉明,汪军,王瑞元.一次性运动对肥胖大鼠脂肪水解调节因素的影响[J].北京体育大学学报,2008,31(11). |
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| 作者姓名： | 田吉明 汪军 王瑞元 |
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| 作者单位： | 山东大学体育学院，北京体育大学 |
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| 基金项目： | 体育总局群众体育科技项目 |
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| 摘 要： | 研究一次性运动对肥胖大鼠脂肪组织HSL、perilipin蛋白含量及脂肪水解的影响。方法:116只3周龄SD雄性大鼠,110只给予10周高脂饲料,建立高脂饮食诱导肥胖大鼠模型。随机分为:安静对照组,运动后即刻、1、31、2和24 h组。用化学法测试血清FFA和甘油、脂肪组织HSL活性;用Western Blotting法测试HSL和perilipin蛋白表达。结果:与对照组相比,运动后FFA和甘油、HSL活性和含量出现不同程度升高;而perilipin含量除即刻组出现显著升高外,其它各组均显著降低。结论:一次性运动导致肥胖大鼠运动后脂解增加,可能与其脂肪组织HSL活性、HSL蛋白含量上调及perilipin蛋白含量下调有关。
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| 关 键 词： | 肥胖 perilipin 脂肪酶 脂肪水解 |
Effects of a Single Bout of Exercise on Regulatory Factors of Obese Rats' Lipolysis |
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| TIAN ji-ming,WANG jun,WANG rui-yuan.Effects of a Single Bout of Exercise on Regulatory Factors of Obese Rats'''' Lipolysis[J].Journal of Beijing Sport University,2008,31(11). |
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| Authors: | TIAN ji-ming WANG jun WANG rui-yuan |
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| Institution: | TIAN ji-ming1,WANG jun2,WANG rui-yuan2 |
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| Abstract: | To research the effects of a single bout of exercise on obese rats' adipose tissue hormone sensitive lipase(HSL) and perilipin protein content as well as lipolysis.Methods: 116 3-week-age Sprange-Dawley male rats,110 rats of all are fed with 10-week high-fat diets to establish high-fat diet-induced obese rats.Then,the obese rats are randomly divided into non-exercise control group (EC),immediate post-exercise group(E0) as well as 1(E1),3(E3),12(E12),and 24(E24) hours after exercise group.The concentrations of serum free fatty acids(FFA),serum glycerol,adipose tissue HSL activity of obese rats are tested using chemical method.Then the HSL and perilipin protein content of rats adipose tissue are tested with Western Blotting method.Results: Compared with EC group,after a single bout of exercise,the serum FFA and serum glycerol concentrations,adipose tissue HSL activity,HSL protein content are remarkably elevated at different times.However,the adipose tissue perilipin content is only higher immediately after a single bout of exercise,and significantly lower at other times,too.Conclusions: A single bout of exercise may result in the increment of obese rats' lipolysis during and after exercise.And the mechanisms of lipolysis rising may be involved in the upregulation of its adipose tissue HSL activity,HSL protein content as well as the downregulation of perilipin protein content. |
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| Keywords: | obesity perilipin lipase lipolysis |
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