| 洱海水华铜绿微囊藻生长特性的初步研究 |
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| 引用本文： | 倪兆林,王涛,张玲,林丽佳,杨冠英,张义,申元英.洱海水华铜绿微囊藻生长特性的初步研究[J].大理师专学报,2013(4):48-50. |
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| 作者姓名： | 倪兆林 王涛 张玲 林丽佳 杨冠英 张义 申元英 |
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| 作者单位： | [1]大理学院公共卫生学院,云南大理671000 [2]大理学院基础医学院,云南大理671000 |
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| 基金项目： | 云南省教育厅科学研究基金资助项目（2012J045）; 大理学院洱海保护研究专项课题基金资助项目（KYRH201005） |
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| 摘 要： | 目的：分离纯化出洱海主要的优势铜绿微囊藻并观察其生长规律及特性。方法：采用水滴-涂布平板法分离纯化铜绿微囊藻,采用90%的丙酮提取叶绿素,酶标仪测定其含量,用显微镜计数藻细胞的个数。结果：铜绿微囊藻细胞密度与OD665呈直线关系,R2=0.993 1,藻细胞的增长速率在第6天达到最大值为0.524,其叶绿素及类胡萝卜素的含量随时间的变化而逐步升高。结论：此铜绿微囊藻的生长符合＂S＂型曲线,藻细胞密度与吸光度成线性相关,其生长速率在第6天达到最大。
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| 关 键 词： | 洱海 铜绿微囊藻 分离 生长曲线 叶绿素a |
A Study on the Growth and Some Physiological Characteristics of Microcystis aeruginosa Isolated from Erhai Lake |
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| NI Zhaolin,WANG Tao,ZHANG Ling,LIN Lijia,YANG Guanying,ZHANG Yi,SHEN Yuanying.A Study on the Growth and Some Physiological Characteristics of Microcystis aeruginosa Isolated from Erhai Lake[J].Journal of Dali Teachers College,2013(4):48-50. |
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| Authors: | NI Zhaolin WANG Tao ZHANG Ling LIN Lijia YANG Guanying ZHANG Yi SHEN Yuanying |
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| Institution: | 1. College of Public Health, Dali University, Dali, Yunnan 671000, China; 2. Pre-clinical College, Dali University, Dali, Yunnan 671000, China ) |
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| Abstract: | Objective： To study on the growth law and some physiological characteristics of Microcystis aeruginosa isolated from Erhai lake. Methods： Purified Microcystis aeruginosa was separated with water droplets method and the spread plate method. Microscopic was used to count the density of algal cells;chlorophyll and carotenoid were extracted with 90% acetone and ELIASA determination of its content. Results： Microcystis aeruginosa cells density was in a linear relationship with OD~, R2 =0.993 1 ;algal cell growth rate in sixth days reached a maximum of 0.524;chlorophyll and carotenoid content increased gradually over time. Conclusion： This Microcystis aeruginosa growth is in line with the ＂S＂ shaped curve; cell density and absorbance is linearly related and the growth rate of the maximum on the sixth day. |
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| Keywords: | Erhai take Microcystis aerug/nosa isolate growth curve chlorophyll a |
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