| 食用仙人掌的组织培养研究初报 |
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| 引用本文： | 秦静远,李鹤荣,范学科,杨淑慎.食用仙人掌的组织培养研究初报[J].杨凌职业技术学院学报,2003,2(1):12-13,16. |
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| 作者姓名： | 秦静远 李鹤荣 范学科 杨淑慎 |
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| 作者单位： | 1. 杨凌职业技术学院,陕西,杨凌,712100
2. 西北农林科技大学,陕西,杨凌,712100 |
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| 摘 要： | 以食用仙人掌的茎片为外植体，研究了食用仙人掌离体快繁的若干影响因素。结果表明，幼嫩的茎片较成熟的茎片易于进行表面消毒。适宜的培养基为：侧芽诱导MS＋6－BA2.0mg/L NAA0.1mg/L，继代与增殖MS＋6－BA2.0mg/L NAA0.1mg/L，培养21d，增殖倍数6．9，生根培养基为1／2 MS＋NAA0.1mg/L，称载基质为泥碳：珍珠岩＝1：1，成活率98％。
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| 关 键 词： | 食用仙人掌 组织培养 培养基 泥碳 外植体 试管苗 |
| 文章编号： | 1671-9131(2003)01-0012-02 |
Preliminary Research on Tissue Culture of Opuntia milpa alta haw |
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| QIN Jing-yuan ,LI He-rong ,FAN Xue-ke ,YANG Shu-shen.Preliminary Research on Tissue Culture of Opuntia milpa alta haw[J].Journal of Yangling Vocational & Technical College,2003,2(1):12-13,16. |
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| Authors: | QIN Jing-yuan LI He-rong FAN Xue-ke YANG Shu-shen |
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| Institution: | QIN Jing-yuan 1,LI He-rong 1,FAN Xue-ke 1,YANG Shu-shen 2 |
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| Abstract: | With the shoots as explants,the effects of different factors on fast in vitro reproduction of Opuntia milpa alta haw are examined. The results showed that the young shoots were easier to be disinfected than the old shoots. MS + 6-BA 2.0 mg/L + NAA 0.1mg/L proved to be an ideal medium for lateral bud induction and MS+6-BA 2.0 mg/L +NAA 0.1 mg/L for subculture and micropropagation, and propagation rate after three weeks was 690%. Rooting medium was 1/2MS + NAA 0.1 mg/L.If the transplant medium contained peat-lite substrate (50% peat and 50% pearlite),the survival percentage was 98%. |
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| Keywords: | opuntia milpa alta haw tissue culture |
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