| 测定维生素A的三种方法比较 |
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| 引用本文： | 钱玲慧,廖佳宇,李亚妮,莫晓帆,马丽,姚彤炜.测定维生素A的三种方法比较[J].实验技术与管理,2012,29(5):43-48. |
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| 作者姓名： | 钱玲慧 廖佳宇 李亚妮 莫晓帆 马丽 姚彤炜 |
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| 作者单位： | 浙江大学药学院,浙江杭州,310058 |
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| 摘 要： | 目的:比较测定维生素A含量的3种不同方法,为维生素A及其制剂的质量研究提供参考。方法:分别采用紫外(UV)三点校正法与正、反相高效液相色谱法(HPLC)测定维生素A含量。UV三点校正法以环己烷为溶剂,按照三点校正法测定;NP-HPLC采用Hypersil SiO2柱(250mm×4.6mm,5μm),流动相为正己烷-异丙醇(99.8∶0.2);RP-HPLC采用C18柱(150mm×4.6mm,5μm),流动相为甲醇-水(97∶3)。结果:UV三点校正法测得VA含量为95.5%,RSD=1.3%(n=5);NP-HPLC为112.5%,RSD=3.2%(n=5);RP-HPLC为102.3%,RSD=1.0%(n=5)。结论:UV三点校正法和RP-HPLC法操作简单易行,NP-HPLC法能较好地控制杂质,但系统稳定性相对较差。
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| 关 键 词： | 维生素A测定 紫外三点校正法 正相高效液相色谱法 反相高效液相色谱法 |
Comparison of three methods on assay for vitamin A: UV, NP-HPLC and RP-HPLC |
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| Qian Linghui
,
Liao Jiayu
,
Li Yani
,
Mo Xiaofan
,
Ma Li
,
Yao Tongwei.Comparison of three methods on assay for vitamin A: UV, NP-HPLC and RP-HPLC[J].Experimental Technology and Management,2012,29(5):43-48. |
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| Authors: | Qian Linghui Liao Jiayu Li Yani Mo Xiaofan Ma Li Yao Tongwei |
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| Institution: | (College of Pharmaceutical Science,Zhejiang University,Hangzhou 310058,China) |
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| Abstract: | Objective To compare three different methods used in vitamin A determination in soft capsule and to provide reference for the assay of vitamin A and its preparations.Method UV,NP-HPLC and RP-HPLC were chosen in this research.Cyclohexane was used as solvent in the triple wavelength method of UV;Column of NP-HPLC was filled with Hypersil SiO2(250×4.6mm,5μm) and the mobile phase consisted of n-hexane and isopropyl alcohol(99.8∶0.2);Column of RP-HPLC was packed with C18(150×4.6mm,5μm) and the mobile phase was methanol-water(97∶3).Results The content of vitamin A determined by UV was 95.5%,RSD=1.3%(n=5);112.5%,RSD=3.2%(n=5)by NP-HPLC and 102.3%,RSD=1.0%(n=5) by RP-HPLC.Conclusion Both UV and RP-HPLC are convenient measurements for vitamin A;NP-HPLC shows advantages in the test of impurities,while its stability is relatively poor. |
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| Keywords: | vitamin A determination triple wavelength method of UV NP-HPLC RP-HPLC |
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