| 药物中盐酸多巴酚丁胺的荧光分析新方法 |
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| 引用本文： | 苗敏,陈亚红.药物中盐酸多巴酚丁胺的荧光分析新方法[J].科教文汇,2014(12):94-95. |
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| 作者姓名： | 苗敏 陈亚红 |
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| 作者单位： | 周口师范学院化学系,河南周口466001 |
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| 基金项目： | 河南省教育厅科学技术研究重点项目（No.14A150015）周口师范学院实验室开放项目（No.K201318）。 |
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| 摘 要： | 利用盐酸多巴酚丁胺对血红蛋白模拟酶催化L-酪氨酸和H2O2体系的抑制作用,建立了酶催化荧光法测定盐酸多巴酚丁胺的新方法。研究了该猝灭反应的最佳实验条件,其猝灭程度与盐酸多巴酚丁胺的浓度呈线性关系。测定的线性范围为5.92×10-7～8.88×10-6mol/L,检出限为1.43×10-9mol/L。对浓度为5.92×10-7mol/L的盐酸多巴酚丁胺溶液进行11次平行测定,其相对标准偏差为3.2%。该方法用于药物中盐酸多巴酚丁胺含量的测定,结果满意。
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| 关 键 词： | 盐酸多巴酚丁胺 荧光法 血红蛋白 |
Spectrofluorimetric Determination of Dobutamine Hy-drochloride in Pharmaceutical |
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| Authors: | Miao Min Chen Yahong |
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| Institution: | Miao Min,Chen Yahong |
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| Abstract: | A high sensitive and simple spectrofluorimetric method for the determination of dobutanmine hydrochloride based on its inhibitory effect on the hemoglobin-catalyzed reaction of H2O2 and L-tyrosine was developed. The concentration of dobutanmine hydrochloride is in linear relationship with the fluorescence quenching （ΔF） of system under the optimal experimental con-ditions. The calibration graph is linear in the range from 5.92í10-7 to 8.88í10-6 mol/L with the detection limit of 1.43í10-9 mol/L. The relative standard deviation for 11 replicate determi-nation of 5.92í10-7 mol/L dobutamine hydrochloride was 3.2%. This method can be used for the determination of dobutanmine hydrochloride in its pharmaceutical formulations and urine with satisfactory results. |
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| Keywords: | dobutamine hydrochloride spectrofluorimetry hemo-globin |
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