| Biodegradation of crude oil by Pseudomonas aeruginosa in the presence of rhamnolipids |
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| 作者姓名： | 张国亮 吴月婷 钱欣平 孟琴 |
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| 作者单位： | School of Materials Science and Chemical Engineering，Zhejiang University，Hangzhou 310027，China Institute of Biological and Environmental Engineering，Zhejiang University of Technology，Hangzhou 310032，China，School of Materials Science and Chemical Engineering，Zhejiang University，Hangzhou 310027，China，School of Materials Science and Chemical Engineering，Zhejiang University，Hangzhou 310027，China，School of Materials Science and Chemical Engineering，Zhejiang University，Hangzhou 310027，China |
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| 基金项目： | Project supported by Science Foundation from China Petroleum andChemical Corporation, China |
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| 摘 要： | INTRODUCTION Oily wastewater, especially from oil field, hasposed a great hazard for terrestrial and marine eco-systems. The traditional treatment of oily wastewater,such as containment and collection using floatingbooms, adsorption by natural or synthetic materials,etc., can not degrade the crude oil thoroughly (Ollis,1992). So far, biodegradation suggests an effectivemethod. During biodegradation, crude oil is used asan organic carbon source by a microbial process,resulting in the brea…
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| 关 键 词： | 生物讲解 鼠李糖 假单胞菌 天然油 发酵处理 甘油 |
| 收稿时间： | 2005-02-21 |
| 修稿时间： | 2005-04-20 |
Biodegradation of crude oil by Pseudomonas aeruginosa in the presence of rhamnolipids |
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| ZHANG Guo-liang,WU Yue-ting,QIAN Xin-ping,MENG Qin.Biodegradation of crude oil by Pseudomonas aeruginosa in the presence of rhamnolipids[J].Journal of Zhejiang University Science,2005,6(8):725-730. |
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| Authors: | ZHANG Guo-liang WU Yue-ting QIAN Xin-ping MENG Qin |
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| Abstract: | The potential biodegradation of crude oil was assessed based on the development of a fermentative process with a strain ofPseudomonas aeruginosa which produced 15.4 g/L rhamnolipids when cultured in a basal mineral medium using glycerol as a sole carbon source. However, neither cell growth nor rhamnolipid production was observed in the comparative culture system using crude oil as the sole carbon source instead. As rhamnolipid, an effective biosurfactant, has been reported to stimulate the biodegradation of hydrocarbons, 1 g/L glycerol or 0.22 g/L rhamnolipid was initially added into the medium to facilitate the biodegradation of crude oil. In both situations, more than 58% of crude oil was degraded and further converted into accumulated cell biomass and rhamnolipids. These results suggest that Pseudomonas aeruginosa could degrade most of crude oil with direct or indirect addition of rhamnolipid. And this conclusion was further supported by another adsorption experiment, where the adsorption capacity of crude oil by killed cell biomass was negligible in comparison with the biologic activities of live cell biomass. |
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| Keywords: | Rhamnolipid Crude oil Biodegradation Pseudomonas aeruginosa |
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