Allograft rejection-related gene expression in the endothelial cells of renal transplantation recipients after cytomegalovirus infection |
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| Authors: | Yang Li Hang Yan Wu-jun Xue Pu-xun Tian Xiao-ming Ding Xiao-ming Pan Xin-shun Feng Xiao-hui Tian He-li Xiang Jun Hou |
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| Institution: | (1) The D. I. Ivanovsky Institute of Virology, Ministry of Health and Social Development of the Russian Federation, 123098, Gamaleya str. 16, Moscow, Russia;(2) Altravita IVF Clinic, 117186, Nagornaya str. 4a, Moscow, Russia;(3) Medical &; Genetic Research Center of Russian Academy of Medical Sciences, 115478, Moscworechye str. 1, Moscow, Russia;(4) Department of Urology, Moscow State Medical Dental University, 127473, Delegatskaya str. 20/1, Moscow, Russia;(5) P.A. Herzen Research Oncological Institute, 125284, 2nd Botkinsky pr. 3, Moscow, Russia |
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| Abstract: | Objective: To explore the effects of cytomegalovirus (CMV) infection on rejection-related gene expression in the endothelial cells of renal transplantation recipients. Methods: Endothelial cells (ECs) were cultured and stimulated by a variety of factors: A, normal control group; B, inactivated human cytomegalovirus (HCMV) infection group; C, HCMV infection group; D, HCMV supematant infection group; and E, ganciclovir HCMV group. Expression of intercellular adhesion molecule-1 (ICAM-1) and major histocompability complex (MHC) class Ⅰ and class Ⅱ antigens was detected by flow cytometry (FCM) and immuno-histochemistry. Results: We found characteristic CMV-infected ECs in this study. There were no significant differences among groups A, B and D (P>0.05). Although the expression levels of ICAM-1 were not significantly different between groups C and E (P>0.05), the ICAM-1 expression in these two groups was significantly higher than that in group A (P<0.05). ICAM-1 expression was detected in groups C and E, while there was no expression in groups A, B and D. Furthermore, there was no significant difference of ICAM-1 mRNA expression between groups C and E (P>0.05). Human leucocyte antigen (HLA)-ABC expression was detected in all the groups, while HLA-DR expression was only detected in groups C and E. There were no significant dif-ferences of HLA-ABC and HLA-DR expression among groups A, B and D (P>0.05). However, the HLA-ABC and HLA-DR expression levels in groups C and D were higher than those of the remaining groups previously reported (P<0.05). Meanwhile, the HLA-ABC and HLA-DR expression levels in group E were lower than those of group C (P<0.05). Conclusion: CMV could up-regulate the expression levels of ICAM-1 and MHC antigens, which was closely related to allograft rejection. |
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| Keywords: | Renal transplantation Cytomegalovirus (CMV) Rejection Intercellular adhesion molecule-1 (ICAM-1) Major histocompability complex (MHC) |
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