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Three dimensional microfluidics with embedded microball lenses for parallel and high throughput multicolor fluorescence detection |
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| Authors: | Y J Fan Y C Wu Y Chen Y C Kung T H Wu K W Huang H J Sheen P Y Chiou |
| Institution: | 1.Mechanical and Aerospace Engineering Department, University of California Los Angeles, Los Angeles, California 90095, USA;2.Institute of Applied Mechanics, National Taiwan University, Taipei 10617, Taiwan;3.Department of Pathology and Laboratory Medicine, University of California Los Angeles, Los Angeles, California 90095, USA;4.Department of Bioengineering, University of California Los Angeles, Los Angeles, California 90095, USA |
| Abstract: | We report a 3D microfluidic device with 32 detection channels and 64 sheath flow channels and embedded microball lens array for high throughput multicolor fluorescence detection. A throughput of 358 400 cells/s has been accomplished. This device is realized by utilizing solid immersion micro ball lens arrays for high sensitivity and parallel fluorescence detection. High refractive index micro ball lenses (n = 2.1) are embedded underneath PDMS channels close to cell detection zones in channels. This design permits patterning high N.A. micro ball lenses in a compact fashion for parallel fluorescence detection on a small footprint device. This device also utilizes 3D microfluidic fabrication to address fluid routing issues in two-dimensional parallel sheath focusing and allows simultaneous pumping of 32 sample channels and 64 sheath flow channels with only two inlets. |
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