| Evaluation of ST13 gene expression in colorectal cancer patients |
| |
| 作者姓名： | DONG Qing-hua |
| |
| 作者单位： | Cancer Institute, Second Affiliated Hospital, School of Medicine, Zhejiang University, Hangzhou 310009, China |
| |
| 摘 要： |
|
| 关 键 词： | 结肠癌 ST13 基因表达 肿瘤组织 治疗方法 |
| 收稿时间： | 2005-07-28 |
| 修稿时间： | 2005-10-04 |
Evaluation of ST13 gene expression in colorectal cancer patients |
| |
| DONG Qing-hua,ZHENG Shu,HU Yue,CHEN Gong-xing,DING Jia-yi.Evaluation of ST13 gene expression in colorectal cancer patients[J].Journal of Zhejiang University Science,2005,6(12):1170-1175. |
| |
| Authors: | DONG Qing-hua ZHENG Shu HU Yue CHEN Gong-xing DING Jia-yi |
| |
| Abstract: | We identified a novel gene ST13 from a subtractive cDNA library of normal intestinal mucosa in 1993, more studies showed that ST13 was a co-chaperone of Hsp70s. Recently we detected the ST13 gene expression in tumor tissue and adjacent normal tissue of the same colorectal cancer patient and investigated ifthe ST13 gene expression might have any prognostic value.Analysis was performed at molecular level by reverse transcfiption-PCR using real-time detection method. We measured two genes simultaneously, ST13 as the target gene and glyceraldehydes-3-phosphate dehydrogenase as a reference gene, in primary colorectal tumor specimens and tumor-adjacent normal mucosa specimens from 50 colorectal cancer patients. The expression levels of the ST13 gene were significantly decreased in primary tumors compared with adjacent mucosa (P＜0.05). But there were no significant differences in the expression of ST13 as compared with different Dukes' stage, tumor differentiation grade, invasion depth, lymph node metastasis and disease-specific survival. |
| |
| Keywords: | ST13 Colorectal cancer Real-time PCR |
| 本文献已被 CNKI 维普 万方数据 等数据库收录！ |
|
