Trapping single human osteoblast-like cells from a heterogeneous population using a dielectrophoretic microfluidic device |
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| Authors: | Rupert S W Thomas Peter D Mitchell Richard O C Oreffo and Hywel Morgan |
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| Institution: | 1The Nano Group, School of Electronics and Computer Science, University of Southampton, Hampshire SO17 1BJ, United Kingdom;2Bone and Joint Research Group, Centre for Human Development, Stem Cells and Regeneration, Developmental Origins of Health and Disease, Institute of Developmental Sciences, University of Southampton Medical School, University of Southampton, Hampshire SO17 1BJ, United Kingdom |
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| Abstract: | We describe a system for the isolation, concentration, separation, and recovery of human osteoblast-like cells from a heterogeneous population using dielectrophoretic ring traps. Cells flowing in a microfluidic channel are immobilized inside an electric field cage using negative dielectrophoresis. A planar ring electrode creates a closed trap while repelling surrounding cells. Target cells are identified by fluorescent labeling, and are trapped as they pass across a ring electrode by an automated system. We demonstrate recovery of small populations of human osteoblast-like cells with a purity of 100%, which in turn demonstrates the potential of such a device for cell selection from a heterogeneous population. |
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