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| 急性跑台运动后大鼠骨骼肌PKB和mTOR蛋白表达的变化 | |
| 引用本文： | 贺道远,曾凡星,叶鸣.急性跑台运动后大鼠骨骼肌PKB和mTOR蛋白表达的变化[J].武汉体育学院学报,2008,42(12):54. |
| 作者姓名： | 贺道远 曾凡星 叶鸣 |
| 作者单位： | 1. 三峡大学,体育学院,湖北,宜昌,443002 2. 北京体育大学,运动人体科学学院,北京,100084 3. 首都体育学院,运动生理生化教研室,北京,100088 |
| 基金项目： | 国家自然科学基金 ， 三峡大学科学基金 |
| 摘 要： | 目的:探讨急性耐力运动对骨骼肌mTOR蛋白表达的影响。方法:SD大鼠分为运动组和对照组。运动组进行坡度5°上坡跑,速度20 m/min、60 min/次的跑台运动后12 h,采用ELISA方法测定腓肠肌IGF-1蛋白表达,western blot测定PKB、mTOR蛋白的表达。结果:1次急性跑台运动后大鼠骨骼肌IGF-1、PKB、mTOR蛋白表达显著上升。结论:急性耐力运动后骨骼肌mTOR信号增加。 |
| 关 键 词： | 耐力运动 翻译调控 哺乳动物雷帕霉素靶蛋白(mTOR) 实验研究 动物实验 大鼠 |
Changes of PKB and mTOR protein expression of skeletal muscle in rats af ter acute treadmill exercise |
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| HE Dao-yuan,ZENG Fan-xing,YE Ming.Changes of PKB and mTOR protein expression of skeletal muscle in rats af ter acute treadmill exercise[J].Journal of Wuhan Institute of Physical Education,2008,42(12):54. | |
| Authors: | HE Dao-yuan ZENG Fan-xing YE Ming |
| Institution: | HE Dao-yuan1,ZENG Fan-xing2,YE Ming3 |
| Abstract: | The purpose of this study was to analyze influences of acute t readmill exercise on mTOR protein expression of skeletal muscle. SD rat s were divided into cont rol group and exercise group. EL ISA and western blot were adopted separately to measure IGF - 1 , PKB ,mTOR protein expression of gast rocnemius muscle in rat after 12 hours following one hour t readmill exercise with 5°incline ,at 20m/ min and 60min/ time. The result s showed that IGF - 1 ,PKB and mTOR protein expression increased significantly in exer- cise group. It was concluded that mTOR signal increased after acute endurance exercise. The data suggest- ed that mTOR might be involved in the regulation of adaptation of muscle growth to exercise. |
| Keywords: | endurance exercise translation regulation mTOR experimental study animal experiment rat |
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