| 用紫外可见分光光度法测定共轭亚油酸的含量 |
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| 作者姓名： | 张亚刚 樊莉 马莉 文彬 吾满江·艾力 |
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| 作者单位： | 1. 中国科学院研究生院, 北京 100039;
2. 中国科学院新疆理化技术研究所, 乌鲁木齐 830011 |
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| 基金项目： | 中国科学院“西部之光”资助项目:共轭亚油酸系列产品的研究与开发(200 2～2004年) |
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| 摘 要： | 采用UV9100型紫外可见分光光度计,测定了共轭亚油酸、共轭亚油酸甲酯、共轭亚油酸三甘酯在不同浓度时在 200～ 300nm的吸光度,绘制出样品在不同浓度时的吸光度曲线,找出适宜的浓度范围;用MicrocalSoftwareOriginVersion 4.0做回归分析,由线性回归给出样品浓度和吸光度的线性关系;利用PEAutosystemXL TurboMass测得样品中共轭亚油酸乙酯的准确浓度,结合回归系数给出了测定样品中共轭亚油酸含量的经验公式.
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| 关 键 词： | 紫外可见分光光度法 共轭亚油酸 十八碳二烯酸 |
| 收稿时间： | 2002-01-13 |
| 修稿时间： | 2002-04-24 |
Quantitation of Conjugated Linoleic Acid by UV Spectrophotometry |
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| Authors: | ZHANG Ya Gang FAN Li MA Li WEN Bin Wumanjiang·ELI |
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| Institution: | 1. Graduate School of Chinese Academy of Sciences, Beijing 100039;
2. Xinjiang Institute of Physical and Chemical Technology, Chinese Academy of Sciences, Urumqi 830011 |
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| Abstract: | Through absorbance measurement of conjugated linoleic acid, methy lester of conjugated linoleic acid and conjug ated linoleic acid trigly cide from 210 ～ 300 nm by UV-9100 spectrophotometer, absorbancy curve of measured samples at different concent ration is plotted.In the appropriate concentration range of samples, linear relationship of absorbancy and samples concent ration is presented by linear regression with Microcal Software Origin Version 4.0 system.The accurate concentration of conjugated linoleic acid is obtained with PE Autosystem XL-T ruboMass.Combined w ith reg ression constant data, emprical formula for the quantitation of conjugated linoleic acid by UV spect rophotometer is given. |
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| Keywords: | ult raviolet-visible spect rophotometry conjugated linoleic acid octadecadienoic acid |
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