Dielectrophoretic field-flow method for separating particle populations in a chip with asymmetric electrodes |
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| Authors: | Ciprian Iliescu Guillaume Tresset and Guolin Xu |
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| Institution: | 1Institute of Bioengineering and Nanotechnology, 31 Biopolis Way, The Nanos 04-01, 138669 Singapore, Singapore;2Laboratoire de Physique des Solides, University Paris-Sud, CNRS, 91405 Orsay, France |
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| Abstract: | This paper presents a field-flow method for separating particle populations in a dielectrophoretic (DEP) chip with asymmetric electrodes under continuous flow. The structure of the DEP device (with one thick electrode that defines the walls of the microfluidic channel and one thin electrode), as well as the fabrication and characterization of the device, was previously described. A characteristic of this structure is that it generates an increased gradient of electric field in the vertical plane that can levitate the particles experiencing negative DEP. The separation method consists of trapping one population to the bottom of the microfluidic channel using positive DEP, while the other population that exhibits negative DEP is levitated and flowed out. Viable and nonviable yeast cells were used for testing of the separation method. |
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