Use of flow cytometry to investigate the cytokine response pattern in infants with respiratory syncytial virus infection and bronchiolitis |
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| Authors: | Email author" target="_blank">Chen?Zhi-minEmail author Du?Li-zhong Mao?Jian-hua Tang?Yong-min |
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| Institution: | (1) The Children's Hospital, College of Medicine, Zhejiang University, 310003 Hangzhou, China |
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| Abstract: | Objective: To investigate the cytokine response pattern (IL-4/IFN-γ) in infants with RSV infections and bronchiolitis during
the acute phase. Methods: Four-color flow cytometry was used to measure intracellular IL-4 and IFN-γ expressions in peripheral
blood CD3+ and CD8+ lymphocytes from RSV-infected and bronchiolitis infants. Serum IL-4 and IFN-γ levels were also determined. Results: RSV-infected
and bronchiolitis infants showed no statistical differences from not-RSV-infected or pneumonia infants and control in the
frequency of IL-4 and IFN-γ expressions in CD3+CD8-lymphocytes, showed no obvious Th1/Th2 imbalance, while IFN-γ was expressed
much more frequently in CD3+CD8+ lymphocytes. Systematically, RSV-infected and bronchiolitis infants showed much lower levels
of serum IL-4 and IL-4/IFN-γ ratios and much higher serum IFN-γ levels than control. Howevr, there were no statistical differences
in the above three indices between RSV-infected and not-RSV infected infants or between bronchiolitis and pneumonia infants,
except that bronchiolitis infants had a higher level of serum IFN-γ than pneumonia infants statistically. Conclusions: There
is not type-2 cytokine response predominance in the acute phase of RSV infection and bronchiolitis. IL-4 production is suppressed
and IFN-γ production upregulated, the latter being most prominent inbronchiolitis infants. |
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| Keywords: | Respiratory syncytial virus (RSV) Bronchiolitis IL-4/IFN-γ Flow cytometry |
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