| 新牧1号苜蓿MvDREB基因的克隆和序列分析 |
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| 引用本文： | 帕尔哈提·阿布都克日木,许磊,朱超,努尔凯麦尔·木拉提,张桦.新牧1号苜蓿MvDREB基因的克隆和序列分析[J].喀什师范学院学报,2014(3):20-23. |
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| 作者姓名： | 帕尔哈提·阿布都克日木 许磊 朱超 努尔凯麦尔·木拉提 张桦 |
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| 作者单位： | [1]新疆农业大学农学院,乌鲁木齐830052 [2]喀什师范学院生物与地理科学系,新疆喀什844008 |
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| 基金项目： | 新疆高技术研究发展项目（201211105） |
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| 摘 要： | 以新牧1号苜蓿为研究材料,采用RT-PCR方法,设计特异引物,从新牧1号苜蓿中克隆获得MvDREB基因cDNA,测序结果该片段全长为651 bp，Genbank登录号为GU073286.生物信息学分析结果表明，该基因序列与紫花苜蓿核苷酸序列相似度达99%,编码216个氨基酸，MvDREB蛋白氨基酸序列分析比对显示该蛋白属于AP2/ERF家族的亲水性核蛋白，蛋白质分子量为24873.1，理论等电点pI为5.90，不稳定参数为45.07.
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| 关 键 词： | 新牧1号苜蓿 MvDREB 序列分析 |
Cloning and Sequence Analysis of MvDREB Gene in Medicago Varia Xinmu No.1 |
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| Paerhati Abudukerimu,XU Lei,ZHU Chao,Nuerkaimaier Mulati,ZHANG Hua.Cloning and Sequence Analysis of MvDREB Gene in Medicago Varia Xinmu No.1[J].Journal of Kashgar Teachers College,2014(3):20-23. |
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| Authors: | Paerhati Abudukerimu XU Lei ZHU Chao Nuerkaimaier Mulati ZHANG Hua |
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| Institution: | 1.College of agriculture, Xinjiang Agricultural University, Urumqi 830052,Xinjiang, China; 2. Departement of Biologic and Geographic Scienees,Kashgar Teachers Cortege, Kashgar 844008,Xinjing, China ) |
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| Abstract: | Using RT-PCR method and designing specific primers, we cloned the cDNA of MvDREB gene in Med-icago.varia.xinmu No.1. the MvDREB（GU073286）is 651 bp long. Bioinformatics analysis results show that, The i-dentity of the nucleotide sequence with Medicago sativa up to 99%, Can encode 216 amino acids. The analysis of the amino acid sequence of MvDREB protein shows that the protein belonging to the family of AP2 / ERF hy-drophilic nucleoprotein. The molecular weight is 24873.1, and theory pI is 5.90, instability parameter is 45.07. |
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| Keywords: | Medicago varia xinmu-1 MvDREB Sequence analysis |
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