| 蒺藜苜蓿液泡膜H~+-ATPase A亚基cDNA的电子克隆与序列分析 |
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| 引用本文： | 孙艳香,李平.蒺藜苜蓿液泡膜H~+-ATPase A亚基cDNA的电子克隆与序列分析[J].河北职业技术学院学报,2009(1). |
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| 作者姓名： | 孙艳香 李平 |
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| 作者单位： | 廊坊师范学院; |
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| 基金项目： | 廊坊师范学院博士专项基金项目(LSZB2OO6O3) |
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| 摘 要： | 根据物种间同源基因相对保守的特点,利用生物信息学的方法,以大豆液泡膜H+-ATPase A亚基基因cDNA序列为信息探针,通过电子克隆得到了蒺藜苜蓿液泡膜H+-ATPase A亚基cDNA,命名为MtVHA-A。该cD-NA长2750bp,其中含5’非翻译区220bp,开放阅读框1875bp,3’非翻译区655bp,其推断的编码产物由624个氨基酸组成,理论分子量为69kDa,与蜜柑、番茄、拟南芥、棉花等植物中液泡膜H+-ATPase A亚基氨基酸序列同源性高达90%以上,且有很高的功能区段保守性。
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| 关 键 词： | 蒺藜苜蓿 液泡膜H+-ATPase A亚基 MtVHA-A |
In Silico Cloning and Analysis of Tonoplast H~+-ATPase A subunit cDNA in Medicago truncatula |
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| SUN Yan-xiang LI Ping.In Silico Cloning and Analysis of Tonoplast H~+-ATPase A subunit cDNA in Medicago truncatula[J].Journal of Hebei Polytechnic,2009(1). |
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| Authors: | SUN Yan-xiang LI Ping |
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| Abstract: | In the present paper,a tonoplast H+ATPase A subunit cDNA,named MtVHA-A was cloned in Medicago truncatula by EST in silico cloning.The cDNA contained 2750 bp with 5'-UTR of 220 bp,ORF region of 1875 bp,3'-UTR of 655 bp,and encoded a 69 kDa protein with 624 amino acid.The deduced amino acid sequence showed more than 90% similarity to homologues from other plants such as Citrus unshiu,Lycopersicon esculentum,Arabidopsis thaliana and Gossypium hirsutum,and highly conserved functional domains of the gene class. |
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| Keywords: | medicago truncatula tonoplast H+-ATPase A subunit MtVHA-A |
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