| 高效液相色谱法测定木瓜中维生素C的含量 |
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| 引用本文： | 姜淑,彭永芳.高效液相色谱法测定木瓜中维生素C的含量[J].昆明师范高等专科学校学报,2009,31(6):102-103. |
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| 作者姓名： | 姜淑 彭永芳 |
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| 作者单位： | 昆明学院化学科学与技术系,云南昆明650031 |
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| 基金项目： | 云南省教育厅科研基金 |
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| 摘 要： | 建立了一种高效液相色谱测定木瓜中维生素C含量的方法．采用0．1％的草酸作为提取剂，色谱中采用Zorbax SB～C18色谱柱，0．1％草酸水作流动相，流速1．0m/min，紫外检测器检测波长261nm．维生素C在20—100μg／mL内有良好的线性关系，线性回归方程为A=73．0542C＋50．6898，相关系数为0．9998，方法回收率为98．81％～102．45％，相对标准偏差等于0．861％．所用测定方法简便、快速，能准确测定木瓜中维生素C的含量．
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| 关 键 词： | 木瓜 高效液相色谱 维生素C |
Determination of Vitamin C in Fructus Chaenomelis by High Performance Liquid Chromatography |
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| JIANG Shu,PENG Yong-fang.Determination of Vitamin C in Fructus Chaenomelis by High Performance Liquid Chromatography[J].Journal of Kunming Teachers College,2009,31(6):102-103. |
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| Authors: | JIANG Shu PENG Yong-fang |
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| Institution: | (Department of Chemistry and Technology, Kunming University, Yunnan Kunming 650031, China) |
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| Abstract: | A practical method of detecting the content of Vitamin C in Fructus ehaenomelis by using HPLC was established. A Zorbax SB -C18 column was used and the mobile phase was 0.1% oxalic acid. The detector was UV with the flow rate being 1.0mL/min and the detection wave length 261 mn in the HPLC system. The standard working curve of Vitamin C had a good liner relation in the concentration ranging from 20 to 100 mg, the liner equation was A = 73. 054 2C ＋ 50. 689 8, and the related coefficient was 0. 999 8. The recoveries were 98.81% - 102.45% and the relatively standard deviation was equal to 0. 861%. This method was simple and fast, and it can exactly detect the content of Vitamin C in Fructus ehaenomelis. |
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| Keywords: | Fructus chaenomelis high performance liquid chromatography （HPLC） vitamin C |
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