| 大鼠脑弥漫性轴索损伤后早期病理学的研究 |
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| 引用本文： | 陈仁辉.大鼠脑弥漫性轴索损伤后早期病理学的研究[J].怀化学院学报,2011,30(2):48-52. |
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| 作者姓名： | 陈仁辉 |
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| 作者单位： | 福建警察学院刑事科学技术系,福建福州,350007 |
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| 基金项目： | 福建省教育厅资助;课题 |
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| 摘 要： | 目的:探讨大鼠脑弥漫性轴索损伤(DAI)后早期轴索病理学改变及c-Fos蛋白的变化,为DAI的早期法医病理学鉴定寻找新的依据.方法:参照Mamarou的打击负荷伤模型,建立大鼠DAI动物模型.运用神经纤维特殊染色法观察轴索的病理学改变,并运用免疫组化SABC法观察c-Fos的变化.结果:嗜银染色于打击后1 h即可观察到...
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| 关 键 词： | 弥漫性轴索损伤 嗜银染色 c-Fos 免疫组织化学 |
A Study on Pathologic Changes in Early Stage of Diffuse Axonal Injury in Rats |
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| CHEN Ren-hui.A Study on Pathologic Changes in Early Stage of Diffuse Axonal Injury in Rats[J].Journal of Huaihua University,2011,30(2):48-52. |
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| Authors: | CHEN Ren-hui |
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| Abstract: | Objective:To explore the changes of c-Fos in early stage of diffuse axonal injury(DAI)in rats so as to find a new evidence for early forensic pathologic diagnosis of DAI.Methods:Based on Marmarou's impact/compression trauma model,the rat model of DAI was established.ImmunohistochemicalSABC method was used to observe the expression of c-Fos,combining special staining of neurol fibers to observe the changes of axons.Results:It was found that swollen and ruptured axon in brain stem at 1h,marked swelling of the axonal severe end at 6 h,axonal retraction ball at 24 h by silver staining after DAI.The expression of c-Fos could be detected at 15 min after injury,peaked at 3 h.The expression of c-Fos was found in wide range but significant in callus and brain stem.The control group showed negative staining.Conclusion:Silver staining is a good way to observe the changes of axons.c-Fos is a sensitive index to DAI and is helpful to diagnose early DAI. |
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| Keywords: | c-Fos |
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