Yangxueqingnao particles inhibit rat vascular smooth muscle cell proliferation induced by lysophosphatidic acid |
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| Authors: | Cai Wei Xu Yi Chen Jun-zhu Huang Shu-ru Lu Zhen-ya and Wang Zhan-kun |
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| Institution: | (1) Department of Internal Medicine, First Affiliated Hospital, School of Medicine, Zhejiang University, 310003 Hangzhou, China |
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| Abstract: | Objective: To observe the effect of Yangxueqingnao particles on rat vascular smooth muscle cell (VSMC) proliferation induced
by lysophosphatidic acid (LPA). Methods: The amount of3H-TdR (3H-thymidine) admixed in cultured rat VSMC was measured and mitogen-activated protein kinase (MAPK) activity and lipid peroxidation
end product malondialdehyde (MDA) content of the VSMC were assayed. Results: 1×10−9, 1×10−8, 1×10−7 mol/L LPA in a concentration dependent manner, induced the amount of3H-TdR admixed, MAP kinase activity, and MDA content of the cultured rat VSMC to increase. However, 5%, 10%, and 15% Yangxueqingnao
serum preincubation resulted in a decrease of 23.0%, 42.0%, and 52.0% (P<0.01) respectively in the amount of3H-TdR admixed, a decline in VSMC MAP kinase activity of 13.9% (P<0.05), 29.6% (P<0.01), and 48.9% (P<0.01) respectively, and also, a decrease in MDA content of VSMC of 19.4%, 24.7%, and 43.2% (P<0.01) respectively, in the 1×10−7 mol/L LPA-treated VSMC. Conclusions: LPA activates the proliferation and lipid peroxidation of VSMC in a concentration dependent
manner. The LPA-induced VSMC proliferation is related to the activity of MAP kinases, enzymes involved in an intracellular
signalling pathway. The results of the present study showed that Yangxueqingnao particles can effectively inhibit LPA-induced
VSMC proliferation, MAP kinase activation, and reduce lipid peroxidative lesion.
Project (No. 491010-W50339) supported by Chinese Traditional Medicine Administration Bureau of Zhejiang Province, China |
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| Keywords: | Yangxueqingnao particles Lysophosphatidic acid Vascular smooth muscle cell Proliferation |
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