| 2,4-D和6-BA对点地梅愈伤组织诱导的影响 |
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| 引用本文： | 聂勇.2,4-D和6-BA对点地梅愈伤组织诱导的影响[J].阿坝师范高等专科学校学报,2006,23(4):126-128. |
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| 作者姓名： | 聂勇 |
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| 作者单位： | 绵阳安县界牌中学,四川,绵阳,622651 |
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| 基金项目： | 四川省杰出青年学科带头人培养计划项目（04ZQ026-047）：四川省科技厅基础项目（03JY029-021-22）. |
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| 摘 要： | 以点地梅(A.umbellate(Lour.)Merr.)无菌苗的嫩叶为外植体,研究了不同质量浓度的2,4-D和6-BA对愈伤组织诱导的影响。结果表明:较高浓度的2,4-D(1.0～2.0 mg/L)单独使用能促进愈伤组织形成,随着2,4-D质量浓度的增加,愈伤组织的形成逐渐变弱;在培养基、2,4-D和6-BA诱导愈伤组织的正交试验中,发现诱导愈伤组织的较优培养基组合为MS+2,4-D 1 mg/L+6-BA 0.4 mg/L,且产生的愈伤组织多为淡绿色、质地疏松、生长快、褐化时间长,适合于点地梅愈伤组织的诱导。
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| 关 键 词： | 点地梅 2 4-D 愈伤组织 |
| 文章编号： | 1008-4142（2006）04-0126-03 |
| 收稿时间： | 2006-09-15 |
| 修稿时间： | 2006年9月15日 |
On the Impact of 2,4-D and 6-BA on the Induction of Androsace Callus Tissues |
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| NIE Yong.On the Impact of 2,4-D and 6-BA on the Induction of Androsace Callus Tissues[J].Journal of Aba Teachers College,2006,23(4):126-128. |
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| Authors: | NIE Yong |
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| Abstract: | Based on taking the sterilized young leaf of Aumbellate(Lour.)Merr.as an explant,this paper makes a study of the influence of 2,4-D and 6-BA in the inducement of callus tissues with different mass concentrations.The result reveals that 2,4-D with higher mass concentrations(1.0～2.0 mg/ L) can promote the formation of callus tissues when used alone.With the increase of mass concentrations for 2,4-D,the formation of the callus tissues is gradually unstrengthening.During the orthogonal experiments of the culture media,2,4-D and 6-BA inductive callus tissues,it is discovered that the better culture media combination for the inductive callus tissues is MS+2,4-D 1 mg/ L+6-BA 0.4 mg/ L,and that the produced callus tissues are mostly greenish,loose in texture,fast in growth,prolonged in browning and suited for the induction of androsace callus tissues. |
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| Keywords: | androsace 2 4-D callus tissues |
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