Evaluation of ovulisa: An indigenous ELISA kit for detection of ovulation |
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| Authors: | G M Sankolli M I Khatkhatay M P Desai D K Pardhe P C Ishrad U M Joshi |
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| Institution: | 1. Institute for Research in Reproduction, Jehangir Merwanji Street, Parel, 440 012, Bombay
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| Abstract: | Aprototype kit for the detection of occurrence of ovulation based on urinary pregnanediol glucuronide estimations by ELISA
was developed in our laboratory. This kit was sent for evaluation at 4 centres in Bombay and Pune, India. The diagnosis of
ovulation/anovulation based on the results of the kit is comparable with that based on reference parameters commonly used
(ultrasonography of ovarian follicles and serum progesterone estimations). The accuracy of the test was 93.5%, specificity
was 87.5% and sensitivity was 95.7%. This rapid indigenous test can be very useful in assessing ovulatory status of the woman
by the clinicians in their offices. |
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