Conversion of renewable substrates for biosurfactant production by Rhizopus arrhizus UCP 1607 and enhancing the removal of diesel oil from marine soil
Institution:
1. Programa de Pós-graduação em Ciências Biológicas, Universidade Federal de Pernambuco, 50670-420 Recife, PE, Brazil;2. Núcleo de Pesquisas em Ciências Ambientais e Biotecnologia, Universidade Católica de Pernambuco, 50050-590 Recife, PE, Brazil;3. Programa de Pós-graduação Rede Nordeste de Biotecnologia, Programa de Pós-graduação Rural de Pernambuco, 52171-900 Recife, PE, Brazil;4. Programa Nacional de Pós-doutorado-PNPD, CAPES, Universidade Católica de Pernambuco, 50050-900 Recife, PE, Brazil;5. Laboratório de Estrutura de Micro-organismos, Instituto de Microbiologia Paulo de Góes, Centro de Ciências da Saúde, Universidade Federal do Rio de Janeiro, Ilha do Fundão, Rio de Janeiro 21941-902, RJ, Brazil;6. Laboratório de Química Biológica de Micro-organismos, Instituto de Microbiologia Paulo de Góes, Centro de Ciências da Saúde, Universidade Federal do Rio de Janeiro, Ilha do Fundão, Rio de Janeiro 21941-902, RJ, Brazil;7. Departamento de Micologia, Universidade Federal de Pernambuco, 50670-901 Recife, PE, Brazil
Abstract:
BackgroundThe use of agro-industrial wastes to produce high value-added biomolecules such as biosurfactants is a promising approach for lowering the total costs of production. This study aimed to produce biosurfactants using Rhizopus arrhizus UCP 1607, with crude glycerol (CG) and corn steep liquor (CSL) as substrates. In addition, the biomolecule was characterized, and its efficiency in removing petroderivatives from marine soil was investigated.ResultsA 22 factorial design was applied, and the best condition for producing the biosurfactant was determined in assay 4 (3% CG and 5% CSL). The biosurfactant reduced the surface tension of water from 72 to 28.8 mN/m and produced a yield of 1.74 g/L. The preliminary biochemical characterization showed that the biosurfactant consisted of proteins (38.0%), carbohydrates (35.4%), and lipids (5.5%). The compounds presented an anionic character, nontoxicity, and great stability for all conditions tested. The biomolecule displayed great ability in dispersing hydrophobic substrates in water, thereby resulting in 53.4 cm2 ODA. The best efficiency of the biosurfactant in removing the pollutant diesel oil from marine soil was 79.4%.ConclusionsThis study demonstrated the ability of R. arrhizus UCP1607 to produce a low-cost biosurfactant characterized as a glycoprotein and its potential use in the bioremediation of the hydrophobic diesel oil pollutant in marine soil.How to cite: Pele MA, Ribeaux DR, Vieira ER, et al. Conversion of renewable substrates for biosurfactant production by Rhizopus arrhizus UCP 1607 and enhancing the removal of diesel oil from marine soil. Electron J Biotechnol 2019;38. https://doi.org/10.1016/j.ejbt.2018.12.003.
