| Magnetic cell sorting and flow cytometry sorting methods for the isolation and function analysis of mouse CD4＋ CD25＋ Treg cells |
| |
| 作者单位： | Department of Renal Transplantation, Center of Nephropathy, the First Affiliated Hospital, Medicine College, Xi＇an Jiaotong University, Xi＇an 710061, China |
| |
| 基金项目： | 国家自然科学基金，the Natural Science Foundation of Shanxi Province，the Science and Technology Key Projects Foundation of Shanxi Province，the Science and Technology Plan Projects Foundation of Xi'an |
| |
| 摘 要： |
|
| 关 键 词： | 流式细胞仪分析 排序方法 细胞分离 CD4 性细胞 T细胞 分选 小鼠 |
| 收稿时间： | 2009 Jul 14 |
Magnetic cell sorting and flow cytometry sorting methods for the isolation and function analysis of mouse CD4+ CD25+ Treg cells |
| |
| Authors: | Hang Yan Chen-guang Ding Pu-xun Tian Guan-qun Ge Zhan-kui Jin Li-ning Jia Xiao-ming Ding Xiao-ming Pan Wu-jun Xue |
| |
| Institution: | 1. Department of Renal Transplantation, Center of Nephropathy, the First Affiliated Hospital, Medicine College, Xi’an Jiaotong University, Xi’an, 710061, China
|
| |
| Abstract: | Objective: In this paper we compared the two methods of cell sorting (magnetic cell sorting and flow cytometry sorting) for the isolation and function analysis of mouse CD4~+ CD25~+ regulatory T (Treg) cells, in order to inform further studies in Treg cell function. Methods: We separately used magnetic cell sorting and flow cytometry sorting to identify CD4~+ CD25~+ Treg cells. After magnetic cell separation, we further used flow cytometry to analyze the purity of CD4~+ CD25~+ Treg cells, trypan blue staining to detect cell viability, and propidium iodide (PI) staining to assess the cell viability. We detected the immune inhibition of CD4~+ CD25~+ Treg cells in the in vitro proliferation experiments. Results: The results showed that compared to flow cytometry sorting, magnetic cell sorting took more time and effort, but fewer live cells were obtained than with flow cytometry sorting. The CD4~+ CD25~+ Treg cells, however, obtained with both methods have similar immunosuppressive capacities. Conclusion: The result suggests that both methods can be used in isolating CD4~+ CD25~+ Treg cells, and one can select the best method according to specific needs and availability of the methodologies. |
| |
| Keywords: | CD4~+ CD25~+ Treg cells Flow cytometry sorting Magnetic cell sorting |
| 本文献已被 维普 万方数据 SpringerLink 等数据库收录！ |
