| 大豆疫霉拮抗细菌B048液体发酵培养基的筛选与发酵条件的优化 |
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| 引用本文： | 汪莞,赵平生,周涛,王子迎.大豆疫霉拮抗细菌B048液体发酵培养基的筛选与发酵条件的优化[J].安徽教育学院学报,2011(6):79-82. |
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| 作者姓名： | 汪莞 赵平生 周涛 王子迎 |
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| 作者单位： | 合肥师范学院生命科学系,安徽合肥,230601 |
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| 基金项目： | [基金项目]国家自然科学基金项目，安徽省优秀青年科技基金项目，教育部留学回国人员科研启动基金 |
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| 摘 要： | 通过单因子实验，筛选得到了适合大豆疫霉拮抗细菌B048液体发酵的最佳碳源为玉米粉，最佳氮源为豆粕，最佳无机盐离子为氯化钠。通过正交实验确定了该培养基的最佳配比为：玉米粉5g／L、豆粕15g／L、氯化钠10g／L。在确定适合该菌株的培养基组分基础上，对其培养条件进行优化，结果表明：初始pH值为6．0，培养时间为48h，转速为180r／min时为该菌株的最佳培养条件。
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| 关 键 词： | 大豆疫霉 B048 培养基 发酵 |
Screening of Liquid Medium and Optimization of Fermentation Conditions for a Bacterium B048 against Phytophthora Sojae |
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| WANG Wan,ZHAO Ping-sheng,ZHOU Tao,WANG Zi-ying.Screening of Liquid Medium and Optimization of Fermentation Conditions for a Bacterium B048 against Phytophthora Sojae[J].Journal of Anhui Institute of Education,2011(6):79-82. |
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| Authors: | WANG Wan ZHAO Ping-sheng ZHOU Tao WANG Zi-ying |
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| Institution: | (Department of life science, Hefei Normal University, Hefei 230061, China) |
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| Abstract: | Single factor test and orthogonal chart were used to optimize the fermentation medium of a bacterium B048. The results indicated that the medium was corn flour 5 g/L, soybean meal 15 g/L, sodium chloride 10 g/L. On the basis of the medium components, the culture conditions were optimized. The re- sults show that the initial pH value is 6, the incubation time is 48 h, and the rotational speed is 180 r/min. |
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| Keywords: | Phytophthora sojae B048 culture medium fermentation |
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