Isolation, PCR based identification, and sensitivity pattern of environmental mycobacteria from leprosy and tuberculosis patients |
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| Authors: | D Saravanakumar N Elangeswaran S Senthilkumar G Vanaja S Kamakshiammal C Chandrasekar C N Deivanayagam Manjula Sritharan V Sritharan |
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| Institution: | (1) Institute of Hansen's disease, Department of Pathology, College of Medicine, The Catholic University of Korea, 505 Banpo-dong, Socho-Gu, 137–701 Seoul, Korea;(2) Institute of Hansen's disease, Department of Pathology, College of Medicine, The Catholic University of Korea, 505 Banpo-dong, Socho-Gu, 137–701 Seoul, Korea;(3) Department of Biostatistics, College of Medicine, The Catholic University of Korea, 505 Banpo-dong, Socho-Gu, 137–701 Seoul, Korea; |
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| Abstract: | We have isolated and identified the biotype of environmental mycobacteria from the expectorate of leprosy patients, their
contacts, their drinking water supply and also from the sputa samples of tuberculosis patients. 78% of the isolates from lepromatous
leprosy patients and their contacts wereMycobacterium fortuitum- chelonae complex (MFC), 9%Mycobacterium avium complex (MAC), 9%Mycobacterium scrofulaceum and 4% wereMycobacterium smegmatis. Among the isolates from tuberculosis patients 63% belonged toM. fortuitum- chelonae complex, 19% toM. avium complex, 12% toMycobacterium Kansasii and 6% toM. smegmatis. All the isolates were multi-drug resistant when tested for sensitivity total of 21 drugs. TheMycobacterium fortuitum-chelonae complex organisms from leprosy contacts were more sensitive to rifampicin than those isolated from lepromatous leprosy and
tuberculosis patients. Among 23 isolates from leprosy patients one isolate was resistant to 20 drugs, one isolate to 17 drugs
and another isolate was resistant to 13 drugs. Among the 18 isolates from drinking water supply six showed resistance to more
than 12 drugs. Polymerase Chain Reaction (PCR) and subsequent hybridisation with specific probes confirmed all the isolated
strains as nontuberculous mycobacteria (Using genus primers and probe sensitivity 100%) and none asM. tuberculosis, suggesting that PCR could be used to rapidly identify mycobacteria at the genus level and to rule out tuberculosis in leprosy
patients at an early stage to decide on appropriate course of therapy. |
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| Keywords: | Environmental mycobacteria leprosy tuberculosis drug resistance |
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