| 尖孢镰刀菌产碱性低温脂肪酶发酵条件的优化 |
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| 引用本文： | 张建国,王蕾.尖孢镰刀菌产碱性低温脂肪酶发酵条件的优化[J].长治学院学报,2012,29(5):1-7. |
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| 作者姓名： | 张建国 王蕾 |
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| 作者单位： | 1. 长治学院生物科学与技术系,山西长治,046000
2. 江南大学生物工程学院,江苏无锡,214122 |
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| 摘 要： | 通过实验对尖孢镰刀菌NC03摇床发酵产脂肪酶的培养基组成和培养条件进行了优化,得出最佳产酶培养基组成配方为：蛋白胨5 g/L,酵母膏6 g/L,NaH2PO43 g/L;橄榄油250 mL/L,吐温80 25 mL/L。最优发酵条件为250 mL的摇瓶装液量50 mL,培养温度30℃,发酵时间84 h。经过优化后发酵液脂肪酶酶活力最高可达到11.32 U/mL,较优化前提高了3.0倍。
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| 关 键 词： | 脂肪酶 培养基 发酵 优化 |
Optimization of Fermentation Conditions for a Low Temperature Lipase Producing Strain |
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| Zhang Jian-guo,Wang Lei.Optimization of Fermentation Conditions for a Low Temperature Lipase Producing Strain[J].Journal of Changzhi University,2012,29(5):1-7. |
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| Authors: | Zhang Jian-guo Wang Lei |
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| Institution: | 1.Department of Biological Sciences and Biotechnology,Changzhi University,Changzhi Shanxi 046000; 2.School of Biotechnology,Jiangnan University,Wuxi Jiangsu 214122) |
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| Abstract: | The effect of single factor on the flask incubation of Fusarium oxysporium NC03 was investigat- ed. The optimum medium for the yield of lipase was that containing peptone 5 g/L, NaH2 PO4 3g/L, olive oil 250 mL/L, emulsifier Tween 80 25mL/L. The strain was cultured under the condition of 30 ℃ for 84 h. The enzyme activity under the optimal condition was 3.0 times of that under the condition before optimized, and reached to 11.32 U/mL |
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| Keywords: | lipase medium fermentation optimization |
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