| 生物分子系统学研究中的RAPD技术 |
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| 引用本文： | 邱小忠,刘莹.生物分子系统学研究中的RAPD技术[J].赣南师范学院学报,1996(6):64-67. |
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| 作者姓名： | 邱小忠 刘莹 |
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| 作者单位： | [1]中山大学生命科学学院 [2]赣南师范学院化学系 |
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| 摘 要： | RAPD技术在Williams等人于1990年首先提出后,在检测DNA多态性方面受到了广泛的应用.RAPD技术以其简单、快速、信息量大等特点渗透在基因诊断,法医检测,生物药品的检验,生物种群关系的研究各个领域.以核酸为指标构建的生物系统进化树,不受主观因素的影响,避免了经典植物系统学的人为影响因素,因而受到广泛应用,在分子系统研究中出现了RFLP,RAPDDNA和RNA测序等分子生物学技术,其中RAPD技术不需要经过DNA分子克隆、分子杂交等繁锁步骤,也不需用放射性同位素显示,并且多态性检出率高,因而受到普遍关注.
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| 关 键 词： | 分子系统学 RAPD技术 PCR技术 |
RAPD TECHNIQUE IN THE STUDY OF MOLECULAR BIOSYSTEMATICS |
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| Qiu xiao zhong.RAPD TECHNIQUE IN THE STUDY OF MOLECULAR BIOSYSTEMATICS[J].Journal of Gannan Teachers' College(Social Science(2)),1996(6):64-67. |
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| Authors: | Qiu xiao zhong |
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| Institution: | Qiu xiao zhong (Department of Biolgy zhong shan University Guangzhuo )Liu ying ( Gan Nan Teacher College ) |
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| Abstract: | Molecular markers such as restriction fragment length polymorphisms (RFLPs)and isozymes have been extensively used for genetic studies and plant identification. The development of the polymeruse chain reaction ( PCR ) has been turned to plants and animals. The radom amplified polymorphic DNA (RAPDs) have been proved useful in genotype identifications and gene mapping in the paper. The genomic DNA-extraction methods as CTAB extration procedure and Edwards extraction procedure is described. The mew procedure fo PCR reaction for RAPD is introduced; The principle and application of RAPDs in Molecular Biosystematics is introduced. |
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| Keywords: | Molecular Biosystematics RARD techniquep PCR techmigue |
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