| Vif基因体外表达载体构建及其在无细胞系统中的表达 |
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| 引用本文： | 汪诚,陈海琴,徐志南,殷秀飞,岑沛霖.Vif基因体外表达载体构建及其在无细胞系统中的表达[J].科技通报,2007,23(3):356-359,367. |
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| 作者姓名： | 汪诚 陈海琴 徐志南 殷秀飞 岑沛霖 |
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| 作者单位： | 1. 浙江大学,生物工程研究所,杭州,310027
2. 浙江养生堂天然药物研究所,杭州,310007 |
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| 摘 要： | 随着-omics（组学）时代的到来,无细胞蛋白质合成系统以具有快速、方便、易于高通量等优点,正在被广泛地研究和应用。本文选取HIV病毒感染因子Vif作为目标蛋白,构建了适宜体外表达的Vif表达载体pIVEX2.4c-Vif,并将其在大肠杆菌无细胞蛋白质合成系统中进行表达,为下一步进行高通量药物筛选奠定了一定的基础。
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| 关 键 词： | 生物合成 无细胞合成系统 |
| 文章编号： | 1001-7119（2007）03-0356-04 |
| 收稿时间： | 2005-11-28 |
| 修稿时间： | 2005-11-28 |
Cloning and Expression of the HIV Protein Vif in Escherichia coli Cell-free System |
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| WANG Cheng,CHEN Hai-qin,XU Zhi-nan,YIN Xiu-fei,CEN Pei-lin.Cloning and Expression of the HIV Protein Vif in Escherichia coli Cell-free System[J].Bulletin of Science and Technology,2007,23(3):356-359,367. |
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| Authors: | WANG Cheng CHEN Hai-qin XU Zhi-nan YIN Xiu-fei CEN Pei-lin |
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| Institution: | 1. Bioengineering Research Institute, Zhejiang University, Hangzhou 310027, China; 2. Zhejiang Yangshengtang Natural Medicines Institute, Hangzhou 310007, China |
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| Abstract: | In the new era of "-omics",the cell-free expression system opens a new strategy in protein expression with advantages of rapid,convenient and high-throughput expression.Cell-free synthesis system enables the direct in vitro expression of protein from template DNA or mRNA.We choose Vif as a target protein,an expression vector pIVEX2.4c-Vif was constructed for the cell-free expression of Vif(virion infectivity factor),and the target protein was synthesized in the batch-mode E.coli cell-free system.This result is the cornerstone of the pharmaceutical high throuhgput process. |
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| Keywords: | HIV Vif(virion infectivity factor) |
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