| 人酸性成纤维细胞生长因子cDNA的表达 |
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| 引用本文： | 吴晓萍,李校堃,郑青,黄亚东,许华,姚成灿.人酸性成纤维细胞生长因子cDNA的表达[J].中山大学学报论丛,2001,21(3):26-30. |
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| 作者姓名： | 吴晓萍 李校堃 郑青 黄亚东 许华 姚成灿 |
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| 作者单位： | 暨南大学医药生物技术研究开发中心,广州,510632 |
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| 摘 要： | 目的：研究人酸性成纤维细胞生长因子（haFGF）cDNA在不同表达系统中的表达，旨在得到高效表达haFGF的表达系统。方法：采用PCR方法扩增人酸性成纤维细胞生长因子(haFGF）cDNA，将该cDNA片段分别插入表达载体pKK223-3及pET3C的表达框架中，筛选得到了重组质粒pKK223-3-haFGF及pET3C-haFGF，分别转化大肠杆菌JM109及BL21（DE3），构建了表达菌株JM109/pKK223-3-haFGF及BL21(DE3)/pET3C-haFGF,用IPTG诱导表达。结果；SDS-PAGE的结果表明，表达菌株BL21(DE3)/pET3C-haFGF能高效表达人酸性成纤维细胞生长因子，而JM109/pKK223-3-haFGF诱导后目标蛋白带不明显。结论：BL21（DE3）/pET3C表达系统更适于表达人酸性成纤维细胞生长因子。
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| 关 键 词： | 人酸性成纤维细胞生长因子 cDNA 表达 |
| 文章编号： | 1007-1792(2001)03-0026-05 |
| 修稿时间： | 2001年4月18日 |
Expression of Human Acidic Fibroblast Growth Factor cDNA in pKK and pET Expression System |
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| WU Xiao-ping,LI Xiao-kun,ZHENC Qing,HUANG Ya-dong,XU Hua,YAO Cheng-can.Expression of Human Acidic Fibroblast Growth Factor cDNA in pKK and pET Expression System[J].Supplement to the Journal of Sun Yatsen University,2001,21(3):26-30. |
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| Authors: | WU Xiao-ping LI Xiao-kun ZHENC Qing HUANG Ya-dong XU Hua YAO Cheng-can |
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| Abstract: | Objective: The expression of human acidic fibroblast growth factor (haFGF) cDNA in pKK and pET expression system was studied. Methods: HaFGF cDNA produced by PCR was separately cloned into the expression vector pKK223-3 and pET3C. The expression of haFGF in JM109/pKK223-3-haFGF and BL21 (DE3) /pET3C-haFGF was induced by IPTG. Results: The expression level of haFGF in BL21 (DE3) /pET3C-haFGF was much higher than that in JM109/pKK223-3-haFGF. Conclusion: The expression system BL21 (DE3) /pET3C is suitable for haFGF expression. |
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| Keywords: | human acidic fibroblast growth factor expression system |
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