| STZ诱导的糖尿病对骨密度和血清睾酮的影响（英文） |
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| 引用本文： | 费聿荣,韦丹,李明,张敏,张晓琳,丁文军,杨建虹.STZ诱导的糖尿病对骨密度和血清睾酮的影响（英文）[J].中国科学院研究生院学报,2007,24(2):217-222. |
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| 作者姓名： | 费聿荣 韦丹 李明 张敏 张晓琳 丁文军 杨建虹 |
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| 作者单位： | 中国科学院研究生院生物系,北京,100049 |
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| 摘 要： | 目的：探讨链脲霉素（STZ）诱导的糖尿病对大鼠骨代谢的影响及机制。材料与方法：雄性Wistar大鼠随机分成3组: 正常组（n = 6），糖尿病组（n = 5）和胰岛素治疗糖尿病组 (n = 5)。大鼠尾静脉一次性注射STZ（50 mg/kg 体重），选择空腹12 h血糖大于12 mmol/L的大鼠为本实验所需的糖尿病模型。治疗组大鼠每天在同一时间给予1.8－2.2U的胰岛素。实验周期为持续32天。采用双能X线骨吸收法（DEXA）测定股骨密度，ELISA法测定血清雄性激素睾酮水平，生化分析仪测定血清碱性磷酸酶、钙、磷浓度。结果：糖尿病大鼠的股骨密度和血清睾酮均显著低于正常组（P < 0.01），血清总碱性磷酸酶明显高于正常组和胰岛素治疗组（P < 0.01）。三组之间血钙和血磷的水平无明显差异。胰岛素治疗后糖尿病大鼠的骨密度、血清睾酮与总碱性磷酸酶得到明显的改善。结论：糖尿病严重影响骨密度，胰岛素缺乏及雄性激素降低是导致糖尿病性骨质疏松重要原因。胰岛素治疗能预防骨流失和提高血清睾酮浓度。
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| 关 键 词： | 骨质疏松 糖尿病 骨密度 睾酮 |
| 文章编号： | 1002-1175(2007)02-0217-06 |
| 修稿时间： | 2006年9月14日 |
Effect of STZ-induced diabetes on bone mineral density and testosterone |
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| FEI Yu-Rong,WEI Dan,LI Ming,ZHANG Min,ZHANG Xiao-Lin,DING Wen-Jun,YANG Jian-Hong.Effect of STZ-induced diabetes on bone mineral density and testosterone[J].Journal of the Graduate School of the Chinese Academy of Sciences,2007,24(2):217-222. |
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| Authors: | FEI Yu-Rong WEI Dan LI Ming ZHANG Min ZHANG Xiao-Lin DING Wen-Jun YANG Jian-Hong |
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| Institution: | Department of Biology, Graduate University of Chinese Academy of Sciences, 19 Yuquan Road, Beijing 100049, P. R. China |
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| Abstract: | Toinvestigate the effect of diabetes mellitus on bone metabolism,bone mineral density(BMD) and serumtestosterone were studied in STZ-induced diabetic male rats.Male Wistar rats were randomly divided into control group(n=6),diabetic group(n=5) and insulin-treated diabetic group(n=5).Diabetes was intravenously induced by injection of streptozotocin(STZ,50mg/kg body weight),confirmed by 12h fasting blood glucose concentration(12mmol/L).The treatment group was subcutaneously injected with protamine zinc insulin(1.8 ~2.2U/animal)at the same time every day.The experiment lasted 32d.Femoral BMD was determined by dual-energy X-ray absorptiometry(DEXA).Serumtestosterone was detected by Enzyme-linked Immunosorbent Assay(ELISA)kit.Total alkaline phosphatase(ALP),calcium (Ca) and phosphorus(P)in serum were measured by bio-machine.Femoral BMDin diabetic group was significantly lower than that in control(P<0.01) but reserved by insulin treatment.Testosterone in serum was also reducedin diabetic rats(P<0.01) and normalized byinsulintreatment.The serum ALP concentration was markedly higher in diabetics(P<0.01) compared with the controls and prevented by insulin treatment.However,no significant differences were observed in serum Ca and P among threegroups.Our results suggest that BMDis seriously affected by diabetes.Insulin deficiency and testosterone decrease are important causes of diabetic osteoporosis.And insulin treatment prevented bone loss and testosterone depression. |
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| Keywords: | osteoporosis diabetes mellitus bone mineral density testosterone |
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