| Cu2＋胁迫对凤眼莲根系活力及Cu2＋-ATPase 活性的影响 |
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| 引用本文： | 王秀丽,李裕红,吴朝金.Cu2＋胁迫对凤眼莲根系活力及Cu2＋-ATPase 活性的影响[J].泉州师范学院学报,2014(6):15-20. |
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| 作者姓名： | 王秀丽 李裕红 吴朝金 |
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| 作者单位： | 1. 厦门大学 环境与生态学院，福建 厦门 361102; 泉州师范学院 化学与生命科学学院，福建 泉州 362000
2. 泉州师范学院 化学与生命科学学院,福建 泉州,362000 |
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| 基金项目： | 国家自然科学基金资助项目 |
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| 摘 要： | 通过水培法研究了不同Cu2＋浓度（0．5,1．0,2．5,5．0,10．0 mg/L）对水生植物凤眼莲Eichhornia crassipes]蛋白质含量、根系活力及Cu2＋-ATP酶活性的影响．结果表明,Cu2＋胁迫对凤眼莲蛋白质含量、根系活力及Cu2＋-ATP酶活性的影响与 Cu2＋胁迫浓度及胁迫时间均有关,在 Cu2＋胁迫时间为1,3,10 d 时,各Cu2＋胁迫浓度下均有利于凤眼莲叶片蛋白质含量的积累．一定浓度和时间的 Cu2＋胁迫使凤眼莲体内蛋白质含量应激积累增多．在Cu2＋胁迫时间为1 d时,随着Cu2＋浓度的增加,凤眼莲根系活力逐渐增大；当胁迫时间达10 d时,根系活力在Cu2＋浓度为1．0 mg/L Cu2＋时达最大,以后逐渐降低．试验表明,随着 Cu2＋浓度的增加,叶片Cu2＋-ATP酶活性在0．5 mg/L时达最大,之后逐渐降低；随Cu2＋浓度的增加根系Cu2＋-ATP酶活性的变化趋势与叶片Cu2＋-ATP酶活性的变化趋势相似．
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| 关 键 词： | Cu2+胁迫 凤眼莲 根系活力 Cu2+-ATP酶 |
Effects of the Stresses of Cu2+ on Activities of Root Activity and Cu2+-ATPase in the Root and Leave of Eichhornia crassipes |
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| WANG Xiu-li,LI Yu-hong,WU Chao-jin.Effects of the Stresses of Cu2+ on Activities of Root Activity and Cu2+-ATPase in the Root and Leave of Eichhornia crassipes[J].Journal of Quanzhou Normal College,2014(6):15-20. |
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| Authors: | WANG Xiu-li LI Yu-hong WU Chao-jin |
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| Institution: | WANG Xiu-li, LI Yu-hong, WU Chao-jin ( 1.College of Environment and Ecology, Xiamen University, Fujian 361102, China 2.School of Chemistry and Life Science,Quanzhou Normal University,Fujian 362000,China) |
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| Abstract: | Water culture method is adopted to research the effects of the stresses of Cu2＋ with different concentrations（0.5,1.0,2.5,5.0,10.0 mg？？L-1 ）on activities of root activity ,protein content and Cu2＋-ATPase in the roots and leaves of Eichhornia crassipes seedlings.The results show that the effects of the stresses of Cu2＋ in short time and low concentrations of Cu2＋ is conducive to the accu-mulation of the protein content in the leaves of Eichhornia crassipes.The effects of the stresses of Cu2＋ in short time and high concentrations of Cu2＋ is conducive to the accumulation of the protein content in the roots of Eichhornia crassipes and the effects of the stresses of Cu2＋,in long time and low concentrations of Cu2＋ is also conducive to the accumulation of the protein content in the roots of Eichhornia crassipes.With the stresses of Cu2＋ in one day,the stress of Cu2＋ can enhance the root ac-tivity with the enlargement of the stresses of Cu2＋.While with the stresses of Cu2＋ in ten days,the root activity reaches the maximum in 1 .0 mg？？L-1 of the concentration of Cu2＋and then gradually re-duces.Along with the increase of Cu2＋ concentration,the Cu2＋-ATPase activity of leave reaches the maximum in 0.5 mg？？L-1 of the concentration of Cu2＋and then gradually reduces.And the variation of Cu2＋-ATPase activity of root is the same as the leave.It can be concluded that the effect of Cu2＋ on root activity,protein content and Cu2＋-ATPase in the roots of Eichhornia crassipes is related to con-centration of Cu2＋ and stress time. |
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| Keywords: | Cu2+stress Eichhornia crassipes root activity Cu2+-ATPase |
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