Visualizing millisecond chaotic mixing dynamics in microdroplets: A direct comparison of experiment and simulation |
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| Authors: | Jiang Liguo Zeng Yan Zhou Hongbo Qu Jianan Y Yao Shuhuai |
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| Institution: | 1Division of Biomedical Engineering, Bioengineering Graduate Program, Hong Kong University of Science and Technology, Hong Kong, China;2Department of Electronic and Computer Engineering, Hong Kong University of Science and Technology, Hong Kong, China;3Department of Mechanical Engineering, Hong Kong University of Science and Technology, Hong Kong, China |
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| Abstract: | In order to fully explore and utilize the advantages of droplet-based microfluidics, fast, sensitive, and quantitative measurements are indispensable for the diagnosis of biochemical reactions in microdroplets. Here, we report an optical detection technique using two-photon fluorescence lifetime imaging microscopy, with an aligning-summing and non-fitting division method, to depict two-dimensional (2D) maps of mixing dynamics by chaotic advection in microdroplets with high temporal and spatial resolution. The mixing patterns of two dye solutions inside droplets were quantitatively and accurately measured. The mixing efficiency in a serpentine droplet mixer was also quantified and compared with the simulation data. The mapped chaotic mixing dynamics agree well with the numerical simulation and theoretical prediction. This quantitative characterization is potentially applicable to the real-time kinetic study of biological and chemical reactions in droplet-based microfluidic systems. |
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